Release of content through mechano-sensitive gates in pressurized liposomes

Martti Louhivuori*, Herre Risselada, Erik van der Giessen, Siewert J. Marrink

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

94 Citations (Scopus)
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Abstract

Mechano-sensitive channels are ubiquitous membrane proteins that activate in response to increasing tension in the lipid membrane. They facilitate a sudden, nonselective release of solutes and water that safeguards the integrity of the cell in hypo-or hyper-osmotic shock conditions. We have simulated the rapid release of content from a pressurized liposome through a particular mechano-sensitive protein channel, MscL, embedded in the liposomal membrane. We show that a single channel is able to relax the liposome, stressed to the point of bursting, in a matter of microseconds. We map the full activation-deactivation cycle of MscL in near-atomic detail and are able to quantify the rapid decrease in liposomal stress as a result of channel activation. This provides a computational tool that opens the way to contribute to the rational design of functional nano-containers.

Original languageEnglish
Pages (from-to)19856-19860
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number46
DOIs
Publication statusPublished - 16-Nov-2010

Keywords

  • coarse-grained
  • molecular dynamics simulation
  • MARTINI force field
  • protein channel
  • drug delivery vehicle
  • MECHANOSENSITIVE CHANNEL MSCL
  • ION-CHANNEL
  • ESCHERICHIA-COLI
  • MEMBRANE TENSION
  • FORCE-FIELD
  • PROTEIN
  • SIMULATIONS
  • MECHANISM

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