Reliability factor for identification of amylolytic enzyme activity in the optimized starch-iodine assay

Aline L.O. Gaenssle; Marc J.E.C. van der Maarel; Edita Jurak

doi:10.1016/j.ab.2020.113696

Reliability factor for identification of amylolytic enzyme activity in the optimized starch-iodine assay

Aline L.O. Gaenssle, Marc J.E.C. van der Maarel, Edita Jurak^*

^*Corresponding author for this work

Bioproduct Engineering

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.

Original language	English
Article number	113696
Number of pages	4
Journal	Analytical Biochemistry
Volume	597
DOIs	https://doi.org/10.1016/j.ab.2020.113696
Publication status	Published - 15-May-2020

Keywords

Amylolytic enzyme
Starch
Iodine assay
Enzyme activity
Mathematical factor
ALPHA-AMYLASE
AMYLOSE

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Reliability factor for identification of amylolytic enzyme activity in theoptimized starch-iodine assayFinal publisher's version, 682 KBLicence: CC BY

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AU - Gaenssle, Aline L.O.

AU - van der Maarel, Marc J.E.C.

AU - Jurak, Edita

PY - 2020/5/15

Y1 - 2020/5/15

N2 - Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.

AB - Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.

KW - Amylolytic enzyme

KW - Starch

KW - Iodine assay

KW - Enzyme activity

KW - Mathematical factor

KW - ALPHA-AMYLASE

KW - AMYLOSE

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

M1 - 113696

ER -

Reliability factor for identification of amylolytic enzyme activity in the optimized starch-iodine assay

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Keywords

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