Abstract
Amylolytic enzymes are a group of proteins degrading starch to its constitutional units. For high-throughput screening, simple yet accurate methods in addition to the reducing ends assays are required. In this article, the iodine assay, a photometric assay based on the intensely colored starch-iodine complex, was adapted to enable accurate and objective differentiation between enzyme and background activity using a newly introduced mathematical factor. The method was further improved by designing a simple setup for multiple time point detection and discussing the applicability of single wavelength measurements.
Original language | English |
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Article number | 113696 |
Number of pages | 4 |
Journal | Analytical Biochemistry |
Volume | 597 |
DOIs | |
Publication status | Published - 15-May-2020 |
Keywords
- Amylolytic enzyme
- Starch
- Iodine assay
- Enzyme activity
- Mathematical factor
- ALPHA-AMYLASE
- AMYLOSE