Response to 'Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita - a multicenter analysis'

M F Jonkman, J M Meijer, G F H Diercks, H H Pas

Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

1 Citation (Scopus)


In a recent retrospective serological study in 95 sera from epidermolysis bullosa acquisita (EBA) patients Schmidt et al. conclude that Col7A-NC1/NC2 ELISA (MBL, Japan) is superior to NC1 ELISA, Western blot and indirect immunofluorescence (IIF) on salt-split skin (SSS) with the highest sensitivity of 97.9%.(1) This sensitivity is an overestimation from a biased sample, since the sera had been preselected from patients with IgG reactivity fitting EBA by at least one of the immune serological assays indirect immunofluorescence, ELISA, and Western blot. The deficiency in study design with an incorrect reference standard creates a bias in estimated test accuracy. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)573
Number of pages1
JournalThe British journal of dermatology
Issue number2
Early online date9-Jan-2018
Publication statusPublished - Feb-2018


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