Reverse Cholesterol Transport Is Increased in Germ-Free Mice-Brief Report

Rima H. Mistry; Henkjan J. Verkade; Uwe J. F. Tietge

doi:10.1161/ATVBAHA.116.308306

Reverse Cholesterol Transport Is Increased in Germ-Free Mice-Brief Report

Rima H. Mistry
, Henkjan J. Verkade
, Uwe J. F. Tietge^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

37 Citations (Scopus)

Abstract

Objective-The intestinal microbiota is emerging as a clinically relevant modulator of atherosclerotic risk. Reverse cholesterol transport (RCT) is an atheroprotective metabolic pathway. How the microbiota impacts RCT has not been investigated. Therefore, the aim of this study was to characterize (cholesterol) metabolism and RCT in germ-free mice compared with conventional mice.

Approach and Results-In chow-fed germ-free mice, plasma cholesterol was unchanged, whereas liver cholesterol content was higher (1.5-fold; P2-fold (P

Conclusions-These data demonstrate that the absence of the intestinal microbiota stimulates RCT >2-fold. Thereby, our results support the importance of intestinal bacteria for metabolic regulation and indicate that specific targeting of the microbiota bears therapeutic potential to prevent and treat cardiovascular disease.

Original language	English
Pages (from-to)	419-422
Number of pages	4
Journal	Arteriosclerosis, thrombosis, and vascular biology
Volume	37
Issue number	3
DOIs	https://doi.org/10.1161/ATVBAHA.116.308306
Publication status	Published - Mar-2017

Keywords

bacteria
cholesterol
liver
microbiota
sterols
BILE-ACID SYNTHESIS
BILIARY STEROL SECRETION
GUT MICROBIOTA
METABOLISM
PHOSPHATIDYLCHOLINE
EFFLUX

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Reverse Cholesterol Transport Is Increased in Germ-Free Mice-Brief Report
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title = "Reverse Cholesterol Transport Is Increased in Germ-Free Mice-Brief Report",

abstract = "Objective-The intestinal microbiota is emerging as a clinically relevant modulator of atherosclerotic risk. Reverse cholesterol transport (RCT) is an atheroprotective metabolic pathway. How the microbiota impacts RCT has not been investigated. Therefore, the aim of this study was to characterize (cholesterol) metabolism and RCT in germ-free mice compared with conventional mice.Approach and Results-In chow-fed germ-free mice, plasma cholesterol was unchanged, whereas liver cholesterol content was higher (1.5-fold; P2-fold (PConclusions-These data demonstrate that the absence of the intestinal microbiota stimulates RCT >2-fold. Thereby, our results support the importance of intestinal bacteria for metabolic regulation and indicate that specific targeting of the microbiota bears therapeutic potential to prevent and treat cardiovascular disease.",

keywords = "bacteria, cholesterol, liver, microbiota, sterols, BILE-ACID SYNTHESIS, BILIARY STEROL SECRETION, GUT MICROBIOTA, METABOLISM, PHOSPHATIDYLCHOLINE, EFFLUX",

author = "Mistry, \{Rima H.\} and Verkade, \{Henkjan J.\} and Tietge, \{Uwe J. F.\}",

note = "{\textcopyright} 2017 American Heart Association, Inc.",

year = "2017",

month = mar,

doi = "10.1161/ATVBAHA.116.308306",

language = "English",

volume = "37",

pages = "419--422",

journal = "Arteriosclerosis, thrombosis, and vascular biology",

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T1 - Reverse Cholesterol Transport Is Increased in Germ-Free Mice-Brief Report

AU - Mistry, Rima H.

AU - Verkade, Henkjan J.

AU - Tietge, Uwe J. F.

PY - 2017/3

Y1 - 2017/3

N2 - Objective-The intestinal microbiota is emerging as a clinically relevant modulator of atherosclerotic risk. Reverse cholesterol transport (RCT) is an atheroprotective metabolic pathway. How the microbiota impacts RCT has not been investigated. Therefore, the aim of this study was to characterize (cholesterol) metabolism and RCT in germ-free mice compared with conventional mice.Approach and Results-In chow-fed germ-free mice, plasma cholesterol was unchanged, whereas liver cholesterol content was higher (1.5-fold; P2-fold (PConclusions-These data demonstrate that the absence of the intestinal microbiota stimulates RCT >2-fold. Thereby, our results support the importance of intestinal bacteria for metabolic regulation and indicate that specific targeting of the microbiota bears therapeutic potential to prevent and treat cardiovascular disease.

AB - Objective-The intestinal microbiota is emerging as a clinically relevant modulator of atherosclerotic risk. Reverse cholesterol transport (RCT) is an atheroprotective metabolic pathway. How the microbiota impacts RCT has not been investigated. Therefore, the aim of this study was to characterize (cholesterol) metabolism and RCT in germ-free mice compared with conventional mice.Approach and Results-In chow-fed germ-free mice, plasma cholesterol was unchanged, whereas liver cholesterol content was higher (1.5-fold; P2-fold (PConclusions-These data demonstrate that the absence of the intestinal microbiota stimulates RCT >2-fold. Thereby, our results support the importance of intestinal bacteria for metabolic regulation and indicate that specific targeting of the microbiota bears therapeutic potential to prevent and treat cardiovascular disease.

KW - bacteria

KW - cholesterol

KW - liver

KW - microbiota

KW - sterols

KW - BILE-ACID SYNTHESIS

KW - BILIARY STEROL SECRETION

KW - GUT MICROBIOTA

KW - METABOLISM

KW - PHOSPHATIDYLCHOLINE

KW - EFFLUX

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DO - 10.1161/ATVBAHA.116.308306

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JO - Arteriosclerosis, thrombosis, and vascular biology

JF - Arteriosclerosis, thrombosis, and vascular biology

IS - 3

ER -

Reverse Cholesterol Transport Is Increased in Germ-Free Mice-Brief Report

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Keywords

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