Ribosomal protein gene RPL9 variants can differentially impair ribosome function and cellular metabolism

Marco Lezzerini, Marianna Penzo, Marie-Francoise O'donohue, Carolina Marques dos Santos Vieira, Manon Saby, Hyung L. Elfrink, Illja J. Diets, Anne-Marie Hesse, Yohann Coute, Marc Gastou, Alexandra Nin-Velez, Peter G. J. Nikkels, Alexandra N. Olson, Evelien Zonneveld-Huijssoon, Marjolijn C. J. Jongmans, GuangJun Zhang, Michel van Weeghel, Riekelt H. Houtkooper, Marcin W. Wlodarski, Roland P. KuiperMarc B. Bierings, Jutte van der Werff ten Bosch, Thierry Leblanc, Lorenzo Montanaro, Jonathan D. Dinman, Lydie Da Costa, Pierre-Emmanuel Gleizes, Alyson W. MacInnes*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    27 Citations (Scopus)
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    Variants in ribosomal protein (RP) genes drive Diamond-Blackfan anemia (DBA), a bone marrow failure syndrome that can also predispose individuals to cancer. Inherited and sporadic RP gene variants are also linked to a variety of phenotypes, including malignancy, in individuals with no anemia. Here we report an individual diagnosed with DBA carrying a variant in the 5 ' UTR of RPL9 (uL6). Additionally, we report two individuals from a family with multiple cancer incidences carrying a RPL9 missense variant. Analysis of cells from these individuals reveals that despite the variants both driving pre-rRNA processing defects and 80S monosome reduction, the downstream effects are remarkably different. Cells carrying the 5 ' UTR variant stabilize TP53 and impair the growth and differentiation of erythroid cells. In contrast, ribosomes incorporating the missense variant erroneously read through UAG and UGA stop codons of mRNAs. Metabolic profiles of cells carrying the 5 ' UTR variant reveal an increased metabolism of amino acids and a switch from glycolysis to gluconeogenesis while those of cells carrying the missense variant reveal a depletion of nucleotide pools. These findings indicate that variants in the same RP gene can drive similar ribosome biogenesis defects yet still have markedly different downstream consequences and clinical impacts.

    Original languageEnglish
    Pages (from-to)770-787
    Number of pages18
    JournalNucleic Acids Research
    Issue number2
    Publication statusPublished - 24-Jan-2020


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