Rif1 Is Required for Resolution of Ultrafine DNA Bridges in Anaphase to Ensure Genomic Stability

Rutger C. C. Hengeveld, H. Rudolf de Boer, Pepijn M. Schoonen, Elisabeth G. E. de Vries, Susanne M. A. Lens*, Marcel A. T. M. van Vugt

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    66 Citations (Scopus)
    167 Downloads (Pure)

    Abstract

    Sister-chromatid disjunction in anaphase requires the resolution of DNA catenanes by topoisomerase II together with Plk1-interacting checkpoint helicase (PICH) and Bloom's helicase (BLM). We here identify Rif1 as a factor involved in the resolution of DNA catenanes that are visible as ultrafine DNA bridges (UFBs) in anaphase to which PICH and BLM localize. Rif1, which during interphase functions downstream of 53BP1 in DNA repair, is recruited to UFBs in a PICH-dependent fashion, but independently of 53BP1 or BLM. Similar to PICH and BLM, Rif1 promotes the resolution of UFBs: its depletion increases the frequency of nucleoplasmic bridges and RPA70-positive UFBs in late anaphase. Moreover, in the absence of Rif1, PICH, or BLM, more nuclear bodies with damaged DNA arise in ensuing G1 cells, when chromosome decatenation is impaired. Our data reveal a thus far unrecognized function for Rif1 in the resolution of UFBs during anaphase to protect genomic integrity.

    Original languageEnglish
    Pages (from-to)466-474
    Number of pages9
    JournalDevelopmental Cell
    Volume34
    Issue number4
    DOIs
    Publication statusPublished - 24-Aug-2015

    Keywords

    • STRAND BREAK REPAIR
    • TOPOISOMERASE-II
    • SPINDLE CHECKPOINT
    • COHESIN REMOVAL
    • END RESECTION
    • REPLICATION
    • MITOSIS
    • CENTROMERE
    • PICH
    • BLM

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