RNA interference in organ transplantation: Next-generation medicine?

Isabel M.A. Brüggenwirth; Paulo N. Martins

doi:10.1016/B978-0-12-819451-5.00008-1

RNA interference in organ transplantation: Next-generation medicine?

Isabel M.A. Brüggenwirth, Paulo N. Martins

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Ischemia-reperfusion injury (IRI) is a major factor in graft quality and organ function after transplantation. Gene silencing through RNA interference (RNAi) may be used to suppress genes related to IRI and modify a graft before transplantation. RNAi is a natural process of posttranscriptional gene regulation that gained a lot of attention after winning the Nobel Prize in Medicine in 2006. Hence, several strategies have been used to deliver siRNA, and pretransplant machine perfusion presents a unique opportunity to deliver siRNA to the target organ during ex situ preservation. This book chapter discusses RNAi in the field of organ transplantation and considers whether this technique may be used as next-generation medicine. It gives a brief overview of the discovery of RNAi and its mechanism of action. In addition, studies using RNAi to target genes related to IRI in liver, kidney, lung, and heart transplantation are discussed.

Original language	English
Title of host publication	Organ Repair and Regeneration
Subtitle of host publication	Preserving Organs in the Regenerative Medicine Era
Editors	Giuseppe Orlando, Shaf Keshavjee
Publisher	Elsevier
Chapter	8
Pages	189-212
Number of pages	24
ISBN (Electronic)	9780128194515
DOIs	https://doi.org/10.1016/B978-0-12-819451-5.00008-1
Publication status	Published - 29-Jan-2021

Keywords

Gene modulation
Ischemia-reperfusion injury
Machine perfusion
RNA interference
Small interfering RNA

Access to Document

10.1016/B978-0-12-819451-5.00008-1

Handle.net

Persistent link

Cite this

@inbook{70a625fae10e45df808953d103a44d70,

title = "RNA interference in organ transplantation: Next-generation medicine?",

abstract = "Ischemia-reperfusion injury (IRI) is a major factor in graft quality and organ function after transplantation. Gene silencing through RNA interference (RNAi) may be used to suppress genes related to IRI and modify a graft before transplantation. RNAi is a natural process of posttranscriptional gene regulation that gained a lot of attention after winning the Nobel Prize in Medicine in 2006. Hence, several strategies have been used to deliver siRNA, and pretransplant machine perfusion presents a unique opportunity to deliver siRNA to the target organ during ex situ preservation. This book chapter discusses RNAi in the field of organ transplantation and considers whether this technique may be used as next-generation medicine. It gives a brief overview of the discovery of RNAi and its mechanism of action. In addition, studies using RNAi to target genes related to IRI in liver, kidney, lung, and heart transplantation are discussed.",

keywords = "Gene modulation, Ischemia-reperfusion injury, Machine perfusion, RNA interference, Small interfering RNA",

author = "Br{\"u}ggenwirth, {Isabel M.A.} and Martins, {Paulo N.}",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd.",

year = "2021",

month = jan,

day = "29",

doi = "10.1016/B978-0-12-819451-5.00008-1",

language = "English",

pages = "189--212",

editor = "Giuseppe Orlando and Shaf Keshavjee",

booktitle = "Organ Repair and Regeneration",

publisher = "Elsevier",

}

TY - CHAP

T1 - RNA interference in organ transplantation

T2 - Next-generation medicine?

AU - Brüggenwirth, Isabel M.A.

AU - Martins, Paulo N.

PY - 2021/1/29

Y1 - 2021/1/29

N2 - Ischemia-reperfusion injury (IRI) is a major factor in graft quality and organ function after transplantation. Gene silencing through RNA interference (RNAi) may be used to suppress genes related to IRI and modify a graft before transplantation. RNAi is a natural process of posttranscriptional gene regulation that gained a lot of attention after winning the Nobel Prize in Medicine in 2006. Hence, several strategies have been used to deliver siRNA, and pretransplant machine perfusion presents a unique opportunity to deliver siRNA to the target organ during ex situ preservation. This book chapter discusses RNAi in the field of organ transplantation and considers whether this technique may be used as next-generation medicine. It gives a brief overview of the discovery of RNAi and its mechanism of action. In addition, studies using RNAi to target genes related to IRI in liver, kidney, lung, and heart transplantation are discussed.

AB - Ischemia-reperfusion injury (IRI) is a major factor in graft quality and organ function after transplantation. Gene silencing through RNA interference (RNAi) may be used to suppress genes related to IRI and modify a graft before transplantation. RNAi is a natural process of posttranscriptional gene regulation that gained a lot of attention after winning the Nobel Prize in Medicine in 2006. Hence, several strategies have been used to deliver siRNA, and pretransplant machine perfusion presents a unique opportunity to deliver siRNA to the target organ during ex situ preservation. This book chapter discusses RNAi in the field of organ transplantation and considers whether this technique may be used as next-generation medicine. It gives a brief overview of the discovery of RNAi and its mechanism of action. In addition, studies using RNAi to target genes related to IRI in liver, kidney, lung, and heart transplantation are discussed.

KW - Gene modulation

KW - Ischemia-reperfusion injury

KW - Machine perfusion

KW - RNA interference

KW - Small interfering RNA

U2 - 10.1016/B978-0-12-819451-5.00008-1

DO - 10.1016/B978-0-12-819451-5.00008-1

M3 - Chapter

AN - SCOPUS:85126415926

SP - 189

EP - 212

BT - Organ Repair and Regeneration

A2 - Orlando, Giuseppe

A2 - Keshavjee, Shaf

PB - Elsevier

ER -