RNA interference in organ transplantation: Next-generation medicine?

Isabel M.A. Brüggenwirth, Paulo N. Martins

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


    Ischemia-reperfusion injury (IRI) is a major factor in graft quality and organ function after transplantation. Gene silencing through RNA interference (RNAi) may be used to suppress genes related to IRI and modify a graft before transplantation. RNAi is a natural process of posttranscriptional gene regulation that gained a lot of attention after winning the Nobel Prize in Medicine in 2006. Hence, several strategies have been used to deliver siRNA, and pretransplant machine perfusion presents a unique opportunity to deliver siRNA to the target organ during ex situ preservation. This book chapter discusses RNAi in the field of organ transplantation and considers whether this technique may be used as next-generation medicine. It gives a brief overview of the discovery of RNAi and its mechanism of action. In addition, studies using RNAi to target genes related to IRI in liver, kidney, lung, and heart transplantation are discussed.

    Original languageEnglish
    Title of host publicationOrgan Repair and Regeneration
    Subtitle of host publicationPreserving Organs in the Regenerative Medicine Era
    EditorsGiuseppe Orlando, Shaf Keshavjee
    Number of pages24
    ISBN (Electronic)9780128194515
    Publication statusPublished - 29-Jan-2021


    • Gene modulation
    • Ischemia-reperfusion injury
    • Machine perfusion
    • RNA interference
    • Small interfering RNA

    Cite this