RNA interference therapeutics in organ transplantation: The dawn of a new era

Isabel M. A. Bruggenwirth, Paulo N. Martins*

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    6 Citations (Scopus)
    28 Downloads (Pure)

    Abstract

    RNA interference (RNAi) is a natural process through which double-stranded RNA molecules can silence the gene carrying the same code as the particular RNA of interest. In 2006, the discovery of RNAi was awarded the Nobel Prize in Medicine and its success has accumulated since. Gene silencing through RNAi has been used successfully in a broad range of diseases, and, more recently, this technique has gained interest in the field of organ transplantation. Here, genes related to ischemia-reperfusion injury (IRI) or graft rejection may be silenced to improve organ quality after transplantation. Several strategies have been used to deliver siRNA, and pretransplant machine perfusion presents a unique opportunity to deliver siRNA to the target organ during ex situ preservation. In this review, the potential of RNAi in the field of organ transplantation will be discussed. A brief overview on the discovery of RNAi, its mechanism, and limitations are included. In addition, studies using RNAi to target genes related to IRI in liver, kidney, lung, and heart transplantation are discussed.

    Original languageEnglish
    Number of pages11
    JournalAmerican Journal of Transplantation
    Early online date4-Nov-2019
    DOIs
    Publication statusPublished - 1-Dec-2019

    Keywords

    • ex situ machine perfusion
    • ex vivo perfusion
    • gene editing
    • gene silencing
    • gene therapy
    • graft modulation
    • graft pretreatment
    • graft treatment
    • ischemia-reperfusion injury (IRI)
    • miRNA
    • small interfering RNA (siRNA)
    • organ preservation
    • organ transplantation
    • regenerative medicine
    • rejection
    • RNA interference
    • RNA therapeutics
    • shRNA
    • siRNA
    • transplant
    • translational research
    • science
    • ISCHEMIA-REPERFUSION INJURY
    • COLD PRESERVATION
    • DONOR ORGANS
    • LIVER
    • SIRNA
    • EXPRESSION
    • CASPASE-3
    • PROTECTS
    • INDUCTION
    • POTENT

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