RNA structure probing, dynamics, and folding

RNA, a pivotal orchestrator of basically every cellular process, heavily relies on its structural conformation to perform its diverse functions. Recent advancements in high-throughput sequencing-based RNA structure mapping techniques have catalyzed a revolution in our ability to investigate the structural landscapes of entire cellular transcriptomes in living cells. These innovative approaches collectively illuminated the previously underestimated intricacies of the RNA structurome, further uncovering dynamic and finely tuned networks of alternative intramolecular and intermolecular interactions. In this chapter I will explore these methodologies, discuss their limitations, and elucidate how they can be harnessed for the exploration of interactions between RNA and small molecules, with the ultimate goal of advancing RNA-focused therapeutic strategies.