@inproceedings{548d3bccd80248e481ef1e10a027f9bb,
title = "Role for CaMKII Inhibition in Rheumatoid Arthritis Effects on HIF-1-Induced VEGF Production by Rheumatoid Synovial Fibroblasts",
abstract = "Background and aims: The present study was designed to investigate the effects of a novel CaMKII inhibitor SMP-114, which was effective in a collagen-induced arthritis model in rats, on rheumatoid synovial fibroblasts. Methods: Rheumatoid synovial fibroblasts (RSF) were cultured under pro-inflammatory (IL-1 beta stimulation) and hypoxic conditions. Protein and mRNA expression of HIF-1 alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed. Results: Stimulation under hypoxic conditions induced higher expression of HIF-1 alpha and VEGF mRNA than treatment with either IL-1 beta or hypoxia alone. However, incubation with a specific CaMKII inhibitor did not result in reduced VEGF production in RSF. Conclusions: CaMKII activation has been reported to be involved in HIF-1 alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.",
keywords = "CaMKII, synovial fibroblast, VEGF, PROTEIN-KINASE-II, HYPOXIA, ACTIVATION, ANGIOGENESIS, MACROPHAGES, EXPRESSION, CYTOKINE, CELLS",
author = "Johanna Westra and Elisabeth Brouwer and Erwin Bouwman and {Doornbos-van der Meer}, Berber and Posthumus, {Marcel D.} and {van Leeuwen}, {Miek A.} and Limburg, {Pieter C.} and Yutaka Ueda and Kallenberg, {Cees G. M.}",
year = "2009",
doi = "10.1111/j.1749-6632.2009.04736.x",
language = "English",
isbn = "978-1-57331-762-7",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing",
pages = "706--711",
editor = "Y Shoenfeld and ME Gershwin",
booktitle = "CONTEMPORARY CHALLENGES IN AUTOIMMUNITY",
note = "6th International Congress on Autoimmunity ; Conference date: 10-09-2008 Through 14-09-2008",
}