Sample preparation for energy dispersive X-ray imaging of biological tissues

Nicole M Pirozzi, Jeroen Kuipers, Ben N G Giepmans*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Abstract

Traditional electron microscopy (EM) can be complemented with analytical EM to increase objective sample information enabling feature identification. Energy dispersive X-ray (EDX) imaging provides semi-quantitative elemental composition of the sample with high spatial resolution (similar to 10nm) in ultrathin sections. However, EDX imaging of biological samples is still challenging as a routine method because many elements are at the detection limit for this technique. Moreover, samples undergo extensive preparation before analysis, which can introduce disruptive X-ray cross-talk or artifacts. EDX data can, for instance, be skewed by (i) osmium interference with endogenous phosphorus, (ii) chlorine present in EPONembedded tissues, (iii) lead interference with endogenous sulfur, and (iv) potential spectral overlaps with grid material, contrast agents, and the in-microscope sample holder. Here, we highlight how to circumvent these potential pitfalls and outline how we approach sample preparation and analysis for detection of different elements of interest. Utilization of wellconsidered a priori sample preparation techniques will best ensure conclusive EDX experiments.

Original languageEnglish
Title of host publicationCORRELATIVE LIGHT AND ELECTRON MICROSCOPY IV
EditorsT MullerReichert, P Verkade
PublisherAcademic Press
Chapter5
Pages89-114
Number of pages26
Volume162
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Cell Biology
PublisherELSEVIER ACADEMIC PRESS INC
Volume162
ISSN (Print)0091-679X

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