SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

Jeroen E. van Schaik; Anna C. Muller Kobold; Bernard F. A. M. van der Laan; Bert van der Vegt; Bettien M. van Hemel; Boudewijn E. C. Plaat

doi:10.1177/01945998221102870

SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

Jeroen E. van Schaik^*, Anna C. Muller Kobold, Bernard F. A. M. van der Laan, Bert van der Vegt, Bettien M. van Hemel, Boudewijn E. C. Plaat

^*Corresponding author for this work

Man, Biomaterials and Microbes (MBM)

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

OBJECTIVE: To determine the diagnostic value of measuring squamous cell carcinoma antigen (SCC-Ag) and cancer antigen 15-3 (CA15-3) concentrations in fine-needle aspiration (FNA) samples for the detection of squamous cell carcinoma (SCC) metastases in cervical lymph nodes.

STUDY DESIGN: A prospective study with patients consecutively included between November 2018 and May 2021.

SETTING: A tertiary head and neck oncologic center.

METHODS: Out of 138 patients, SCC-Ag concentrations were analyzed in 168 FNA cervical lymph node samples and CA15-3 in 152 samples. Results were compared with FNA cytology (FNAC) or definitive histology to establish sensitivity and specificity rates.

RESULTS: For the detection of cervical SCC lymph node metastases, SCC-Ag measurement had an 89.4% sensitivity and 79.3% specificity at a cutoff concentration of 0.1 µg/L. Measurement of CA15-3 concentration in addition to SCC-Ag concentration did not lead to improved accuracy for the detection of SCC. In histology-confirmed cases, FNAC had an 80.0% sensitivity and 100% specificity, as opposed to 93.3% and 57.1%, respectively, for SCC-Ag.

CONCLUSION: Measurement of SCC-Ag concentration for detection of SCC lymph node metastases has a sensitivity at least comparable to FNAC and could be used as a relatively cheap screening tool in samples with nondiagnostic or indeterminate FNAC results or when multiple lymph nodes are sampled. However, SCC-Ag in FNA samples has a lower specificity than FNAC assessed by pathologists experienced in head and neck oncology. Addition of CA15-3 measurement did not lead to improved accuracy.

Original language	English
Article number	01945998221102870
Pages (from-to)	407-412
Number of pages	6
Journal	Otolaryngology - Head and Neck Surgery
Volume	168
Issue number	3
Early online date	31-May-2022
DOIs	https://doi.org/10.1177/01945998221102870
Publication status	Published - Mar-2023

Keywords

SCC-Ag
mucin 1
fine-needle aspiration
tumor marker
squamous cell carcinoma
lymph node
HEAD
CYTOLOGY
LESIONS
MUC1

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van Schaik, J. E., Muller Kobold, A. C., van der Laan, B. F. A. M., van der Vegt, B., van Hemel, B. M., & Plaat, B. E. C. (2023). SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis. Otolaryngology - Head and Neck Surgery, 168(3), 407-412. Article 01945998221102870. https://doi.org/10.1177/01945998221102870

@article{e4d7448565374c40932de907eaf7bbe1,

title = "SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis",

abstract = "OBJECTIVE: To determine the diagnostic value of measuring squamous cell carcinoma antigen (SCC-Ag) and cancer antigen 15-3 (CA15-3) concentrations in fine-needle aspiration (FNA) samples for the detection of squamous cell carcinoma (SCC) metastases in cervical lymph nodes.STUDY DESIGN: A prospective study with patients consecutively included between November 2018 and May 2021.SETTING: A tertiary head and neck oncologic center.METHODS: Out of 138 patients, SCC-Ag concentrations were analyzed in 168 FNA cervical lymph node samples and CA15-3 in 152 samples. Results were compared with FNA cytology (FNAC) or definitive histology to establish sensitivity and specificity rates.RESULTS: For the detection of cervical SCC lymph node metastases, SCC-Ag measurement had an 89.4% sensitivity and 79.3% specificity at a cutoff concentration of 0.1 µg/L. Measurement of CA15-3 concentration in addition to SCC-Ag concentration did not lead to improved accuracy for the detection of SCC. In histology-confirmed cases, FNAC had an 80.0% sensitivity and 100% specificity, as opposed to 93.3% and 57.1%, respectively, for SCC-Ag.CONCLUSION: Measurement of SCC-Ag concentration for detection of SCC lymph node metastases has a sensitivity at least comparable to FNAC and could be used as a relatively cheap screening tool in samples with nondiagnostic or indeterminate FNAC results or when multiple lymph nodes are sampled. However, SCC-Ag in FNA samples has a lower specificity than FNAC assessed by pathologists experienced in head and neck oncology. Addition of CA15-3 measurement did not lead to improved accuracy.",

keywords = "SCC-Ag, mucin 1, fine-needle aspiration, tumor marker, squamous cell carcinoma, lymph node, HEAD, CYTOLOGY, LESIONS, MUC1",

author = "{van Schaik}, {Jeroen E.} and {Muller Kobold}, {Anna C.} and {van der Laan}, {Bernard F. A. M.} and {van der Vegt}, Bert and {van Hemel}, {Bettien M.} and Plaat, {Boudewijn E. C.}",

year = "2023",

month = mar,

doi = "10.1177/01945998221102870",

language = "English",

volume = "168",

pages = "407--412",

journal = "Otolaryngology - Head and Neck Surgery",

issn = "0194-5998",

publisher = "SAGE Publications Inc.",

number = "3",

}

SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis. / van Schaik, Jeroen E.; Muller Kobold, Anna C.; van der Laan, Bernard F. A. M. et al.
In: Otolaryngology - Head and Neck Surgery, Vol. 168, No. 3, 01945998221102870, 03.2023, p. 407-412.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases

T2 - A Prospective Analysis

AU - van Schaik, Jeroen E.

AU - Muller Kobold, Anna C.

AU - van der Laan, Bernard F. A. M.

AU - van der Vegt, Bert

AU - van Hemel, Bettien M.

AU - Plaat, Boudewijn E. C.

PY - 2023/3

Y1 - 2023/3

N2 - OBJECTIVE: To determine the diagnostic value of measuring squamous cell carcinoma antigen (SCC-Ag) and cancer antigen 15-3 (CA15-3) concentrations in fine-needle aspiration (FNA) samples for the detection of squamous cell carcinoma (SCC) metastases in cervical lymph nodes.STUDY DESIGN: A prospective study with patients consecutively included between November 2018 and May 2021.SETTING: A tertiary head and neck oncologic center.METHODS: Out of 138 patients, SCC-Ag concentrations were analyzed in 168 FNA cervical lymph node samples and CA15-3 in 152 samples. Results were compared with FNA cytology (FNAC) or definitive histology to establish sensitivity and specificity rates.RESULTS: For the detection of cervical SCC lymph node metastases, SCC-Ag measurement had an 89.4% sensitivity and 79.3% specificity at a cutoff concentration of 0.1 µg/L. Measurement of CA15-3 concentration in addition to SCC-Ag concentration did not lead to improved accuracy for the detection of SCC. In histology-confirmed cases, FNAC had an 80.0% sensitivity and 100% specificity, as opposed to 93.3% and 57.1%, respectively, for SCC-Ag.CONCLUSION: Measurement of SCC-Ag concentration for detection of SCC lymph node metastases has a sensitivity at least comparable to FNAC and could be used as a relatively cheap screening tool in samples with nondiagnostic or indeterminate FNAC results or when multiple lymph nodes are sampled. However, SCC-Ag in FNA samples has a lower specificity than FNAC assessed by pathologists experienced in head and neck oncology. Addition of CA15-3 measurement did not lead to improved accuracy.

AB - OBJECTIVE: To determine the diagnostic value of measuring squamous cell carcinoma antigen (SCC-Ag) and cancer antigen 15-3 (CA15-3) concentrations in fine-needle aspiration (FNA) samples for the detection of squamous cell carcinoma (SCC) metastases in cervical lymph nodes.STUDY DESIGN: A prospective study with patients consecutively included between November 2018 and May 2021.SETTING: A tertiary head and neck oncologic center.METHODS: Out of 138 patients, SCC-Ag concentrations were analyzed in 168 FNA cervical lymph node samples and CA15-3 in 152 samples. Results were compared with FNA cytology (FNAC) or definitive histology to establish sensitivity and specificity rates.RESULTS: For the detection of cervical SCC lymph node metastases, SCC-Ag measurement had an 89.4% sensitivity and 79.3% specificity at a cutoff concentration of 0.1 µg/L. Measurement of CA15-3 concentration in addition to SCC-Ag concentration did not lead to improved accuracy for the detection of SCC. In histology-confirmed cases, FNAC had an 80.0% sensitivity and 100% specificity, as opposed to 93.3% and 57.1%, respectively, for SCC-Ag.CONCLUSION: Measurement of SCC-Ag concentration for detection of SCC lymph node metastases has a sensitivity at least comparable to FNAC and could be used as a relatively cheap screening tool in samples with nondiagnostic or indeterminate FNAC results or when multiple lymph nodes are sampled. However, SCC-Ag in FNA samples has a lower specificity than FNAC assessed by pathologists experienced in head and neck oncology. Addition of CA15-3 measurement did not lead to improved accuracy.

KW - SCC-Ag

KW - mucin 1

KW - fine-needle aspiration

KW - tumor marker

KW - squamous cell carcinoma

KW - lymph node

KW - HEAD

KW - CYTOLOGY

KW - LESIONS

KW - MUC1

U2 - 10.1177/01945998221102870

DO - 10.1177/01945998221102870

M3 - Article

C2 - 35639471

SN - 0194-5998

VL - 168

SP - 407

EP - 412

JO - Otolaryngology - Head and Neck Surgery

JF - Otolaryngology - Head and Neck Surgery

IS - 3

M1 - 01945998221102870

ER -

SCC Antigen Concentrations in Fine-Needle Aspiration Samples to Detect Cervical Lymph Node Metastases: A Prospective Analysis

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