Secondary cataract material collected with a glass cannula: A SEM study

W. L. Jongebloed; G. Van der Veen; F. Dijk; J. G. F. Worst

doi:10.1007/BF00164851

Secondary cataract material collected with a glass cannula: A SEM study

W. L. Jongebloed^*, G. Van der Veen, F. Dijk, J. G. F. Worst

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.

Original language	English
Pages (from-to)	359-364
Number of pages	6
Journal	Documenta ophthalmologica
Volume	75
Issue number	3-4
DOIs	https://doi.org/10.1007/BF00164851
Publication status	Published - Oct-1990

Keywords

SECONDARY CATARACT
LENS-FIBERS
CAPSULE EPITHELIUM
SELECTIVE COLLECTION
GLASS CANNULA
SEM
MORPHOLOGICAL DESCRIPTION

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title = "Secondary cataract material collected with a glass cannula: A SEM study",

abstract = "Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.",

keywords = "SECONDARY CATARACT, LENS-FIBERS, CAPSULE EPITHELIUM, SELECTIVE COLLECTION, GLASS CANNULA, SEM, MORPHOLOGICAL DESCRIPTION",

author = "Jongebloed, {W. L.} and {Van der Veen}, G. and F. Dijk and Worst, {J. G. F.}",

year = "1990",

month = oct,

doi = "10.1007/BF00164851",

language = "English",

volume = "75",

pages = "359--364",

journal = "Documenta ophthalmologica",

issn = "0012-4486",

number = "3-4",

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TY - JOUR

T1 - Secondary cataract material collected with a glass cannula

T2 - A SEM study

AU - Jongebloed, W. L.

AU - Van der Veen, G.

AU - Dijk, F.

AU - Worst, J. G. F.

PY - 1990/10

Y1 - 1990/10

N2 - Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.

AB - Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.

KW - SECONDARY CATARACT

KW - LENS-FIBERS

KW - CAPSULE EPITHELIUM

KW - SELECTIVE COLLECTION

KW - GLASS CANNULA

KW - SEM

KW - MORPHOLOGICAL DESCRIPTION

U2 - 10.1007/BF00164851

DO - 10.1007/BF00164851

M3 - Article

SN - 0012-4486

VL - 75

SP - 359

EP - 364

JO - Documenta ophthalmologica

JF - Documenta ophthalmologica

IS - 3-4

ER -