Seeing is believing? When scanning electron microscopy (SEM) meets clinical dentistry: The replica technique.

Lucas Zago Naves*, David-Alain Gerdolle, Oswaldo Scopin de Andrade, Marco Markus Maria Gresnigt

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    1 Citation (Scopus)
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    In restorative dentistry, the in situ replication of intra-oral situations, is based on a non-invasive and non-destructive scanning electron microscopy (SEM) evaluation method. The technique is suitable for investigation restorative materials and dental hard- and soft-tissues, and its interfaces. Surface characteristics, integrity of interfaces (margins), or fracture analysis (chipping, cracks, etc.) with reliable resolution and under high magnification (from x50 to x5,000). Overall the current study aims to share detailed and reproducible information about the replica technique. Specific goals are: (a) to describe detailed each step involved in producing a replica of an intra-oral situation, (b) to validate an integrated workflow based on a rational sequence from visual examination, to macrophotography and SEM analysis using the replica technique; (c) to present three clinical cases documented using the technique. A compilation of three clinical situations/cases were analyzed here by means the replica technique showing a wide range of possibilities that can be reached and explored with the described technique. This guidance document will contribute to a more accurate use of the replica technique and help researchers and clinicians to understand and identify issues related to restorative procedures under high magnification.

    Original languageEnglish
    Pages (from-to)1118-1123
    Number of pages6
    JournalMicroscopy Research and Technique
    Issue number9
    Publication statusPublished - 1-Sep-2020


    • clinical dentistry
    • magnification
    • replica technique
    • scanning electron microscopy
    • in vivo evaluation

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