Selection, Addiction and Catalysis: Emerging Trends for the Incorporation of Noncanonical Amino Acids into Peptides and Proteins in Vivo

Clemens Mayer*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

5 Citations (Scopus)
131 Downloads (Pure)

Abstract

Expanding the genetic code of organisms by incorporating non‐canonical amino acids (ncAAs) into target proteins through the suppression of stop codons in vivo has profoundly impacted how we perform protein modification or detect proteins and their interaction partners in their native environment. Yet, with genetic code expansion strategies maturing over the past 15 years, new applications that make use – or indeed repurpose – these techniques are beginning to emerge. This concept article highlights three of these developments: (1) the incorporation of ncAAs for the biosynthesis and selection of bioactive macrocyclic peptides with novel ring architectures, (2) synthetic biocontainment strategies based on the addiction of microorganisms to ncAAs, and (3) enzyme design strategies, in which ncAAs with unique functionalities enable the catalysis of new‐to‐nature reactions. Key advances in all three areas are presented and potential future applications discussed.
Original languageEnglish
Pages (from-to)1357-1364
Number of pages8
JournalChemBioChem
Volume20
Issue number11
Early online date8-Jan-2019
DOIs
Publication statusPublished - 3-Jun-2019

Keywords

  • MACROCYCLIC PEPTIDES
  • RIBOSOMAL SYNTHESIS
  • GENETIC-CODE
  • ARTIFICIAL METALLOENZYMES
  • NUCLEOPHILIC CATALYSIS
  • DESIGN
  • GENERATION
  • ORGANISMS
  • EVOLUTION
  • HYBRIDS

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