Selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site

Matthias Gautschi; Jaap J. Beintema

doi:10.1007/s10528-008-9162-x

Selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site

Matthias Gautschi
, Jaap J. Beintema^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn-Met-Thr at residues 34-36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.

Original language	English
Pages (from-to)	446-450
Number of pages	5
Journal	Biochemical genetics
Volume	46
Issue number	7-8
DOIs	https://doi.org/10.1007/s10528-008-9162-x
Publication status	Published - Aug-2008

Keywords

bovine
glycoprotein
molecular evolution
ribonuclease
ruminants

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Selection against glycosylation in ruminant pancreatic ribonucleases by replacementsFinal publisher's version, 202 KBLicence: CC BY-NC

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title = "Selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site",

abstract = "A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn-Met-Thr at residues 34-36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.",

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AU - Gautschi, Matthias

AU - Beintema, Jaap J.

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N2 - A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn-Met-Thr at residues 34-36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.

AB - A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn-Met-Thr at residues 34-36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.

KW - bovine

KW - glycoprotein

KW - molecular evolution

KW - ribonuclease

KW - ruminants

U2 - 10.1007/s10528-008-9162-x

DO - 10.1007/s10528-008-9162-x

M3 - Article

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VL - 46

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EP - 450

JO - Biochemical genetics

JF - Biochemical genetics

IS - 7-8

ER -

Selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site

Abstract

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