Selection strategies for improved biocatalysts

Ykelien L. Boersma; Melloney J. Droge; Wim J. Quax

doi:10.1111/j.1742-4658.2007.05782.x

Selection strategies for improved biocatalysts

Ykelien L. Boersma, Melloney J. Droge, Wim J. Quax^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

58 Citations (Scopus)

Abstract

Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. However, selection of the evolved variants is a critical step, and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries, in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals.

Original language	English
Pages (from-to)	2181-2195
Number of pages	15
Journal	Febs Journal
Volume	274
Issue number	9
DOIs	https://doi.org/10.1111/j.1742-4658.2007.05782.x
Publication status	Published - May-2007

Keywords

cell surface display
directed evolution
enzymes
genetic selection
in vitro compartmentalization
phage display
ribosome display
IN-VITRO COMPARTMENTALIZATION
CELL-SURFACE DISPLAY
DIRECTED PROTEIN EVOLUTION
SUBTILIS LIPASE-A
PHAGE-DISPLAY
ESCHERICHIA-COLI
GENETIC SELECTION
CHEMICAL COMPLEMENTATION
SUBSTRATE-SPECIFICITY
CATALYTIC-ACTIVITY

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Selection strategies for improved biocatalysts
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title = "Selection strategies for improved biocatalysts",

abstract = "Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. However, selection of the evolved variants is a critical step, and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries, in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals.",

keywords = "cell surface display, directed evolution, enzymes, genetic selection, in vitro compartmentalization, phage display, ribosome display, IN-VITRO COMPARTMENTALIZATION, CELL-SURFACE DISPLAY, DIRECTED PROTEIN EVOLUTION, SUBTILIS LIPASE-A, PHAGE-DISPLAY, ESCHERICHIA-COLI, GENETIC SELECTION, CHEMICAL COMPLEMENTATION, SUBSTRATE-SPECIFICITY, CATALYTIC-ACTIVITY",

author = "Boersma, {Ykelien L.} and Droge, {Melloney J.} and Quax, {Wim J.}",

year = "2007",

month = may,

doi = "10.1111/j.1742-4658.2007.05782.x",

language = "English",

volume = "274",

pages = "2181--2195",

journal = "Febs Journal",

issn = "1742-464X",

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T1 - Selection strategies for improved biocatalysts

AU - Boersma, Ykelien L.

AU - Droge, Melloney J.

AU - Quax, Wim J.

PY - 2007/5

Y1 - 2007/5

N2 - Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. However, selection of the evolved variants is a critical step, and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries, in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals.

AB - Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. However, selection of the evolved variants is a critical step, and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries, in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals.

KW - cell surface display

KW - directed evolution

KW - enzymes

KW - genetic selection

KW - in vitro compartmentalization

KW - phage display

KW - ribosome display

KW - IN-VITRO COMPARTMENTALIZATION

KW - CELL-SURFACE DISPLAY

KW - DIRECTED PROTEIN EVOLUTION

KW - SUBTILIS LIPASE-A

KW - PHAGE-DISPLAY

KW - ESCHERICHIA-COLI

KW - GENETIC SELECTION

KW - CHEMICAL COMPLEMENTATION

KW - SUBSTRATE-SPECIFICITY

KW - CATALYTIC-ACTIVITY

U2 - 10.1111/j.1742-4658.2007.05782.x

DO - 10.1111/j.1742-4658.2007.05782.x

M3 - Review article

SN - 1742-464X

VL - 274

SP - 2181

EP - 2195

JO - Febs Journal

JF - Febs Journal

IS - 9

ER -

Selection strategies for improved biocatalysts

Abstract

Keywords

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