TY - JOUR
T1 - Serum cytokine profiling as a diagnostic and prognostic tool in ovarian cancer
T2 - A potential role for interleukin 7
AU - Lambeck, Annechien J. A.
AU - Crijns, Anne P. G.
AU - Leffers, Ninke
AU - Sluiter, Wim J.
AU - ten Hoor, Klaske A.
AU - Braid, Mike
AU - van der Zee, Ate G. J.
AU - Daemen, Toos
AU - Nijman, Hans W.
AU - Kast, W. Martin
PY - 2007/4/15
Y1 - 2007/4/15
N2 - Purpose: To evaluate if serum cytokine levels could be used as diagnostic or prognostic markers in ovarian cancer.Experimental Design: A cytokine bead array was done to simultaneously analyze 14 cytokines in the sera of 187 ovarian cancer patients with complete clinicopathologic data and follow-up, 45 patients with benign ovarian tumors, and 50 healthy controls. Serum levels of the well-known serum tumor marker CA-125 were routinely measured in all patients.Results: Serum levels of CA-125, interleukin 6 (IL-6), IL-7, and IL-10 were elevated in ovarian cancer patients compared with patients with benign ovarian tumors. Analyzing the cytokines in combination with CA-125 showed that a combination of IL-7 and CA-125 serum levels could accurately predict 69% of the ovarian cancer patients, without falsely classifying patients with benign pelvic mass. The cytokines IL-6, IL-7, IL-8, IL-10, monocyte chemotactic protein-1 (MCP-1), and IP-10 and CA-125 were associated with disease-free and overall survival in univariate analysis. In multivariate analysis, IL-7 and IP-10 were independent predictors of overall survival, although after inclusion of the clininopathologic parameters, only stage and residual disease remained as independent predictors of survival.Conclusions: IL-7 levels were found to be strongly associated with ovarian cancer and could be used in combination with CA-125 to distinguish between malignant and benign ovarian tumors.
AB - Purpose: To evaluate if serum cytokine levels could be used as diagnostic or prognostic markers in ovarian cancer.Experimental Design: A cytokine bead array was done to simultaneously analyze 14 cytokines in the sera of 187 ovarian cancer patients with complete clinicopathologic data and follow-up, 45 patients with benign ovarian tumors, and 50 healthy controls. Serum levels of the well-known serum tumor marker CA-125 were routinely measured in all patients.Results: Serum levels of CA-125, interleukin 6 (IL-6), IL-7, and IL-10 were elevated in ovarian cancer patients compared with patients with benign ovarian tumors. Analyzing the cytokines in combination with CA-125 showed that a combination of IL-7 and CA-125 serum levels could accurately predict 69% of the ovarian cancer patients, without falsely classifying patients with benign pelvic mass. The cytokines IL-6, IL-7, IL-8, IL-10, monocyte chemotactic protein-1 (MCP-1), and IP-10 and CA-125 were associated with disease-free and overall survival in univariate analysis. In multivariate analysis, IL-7 and IP-10 were independent predictors of overall survival, although after inclusion of the clininopathologic parameters, only stage and residual disease remained as independent predictors of survival.Conclusions: IL-7 levels were found to be strongly associated with ovarian cancer and could be used in combination with CA-125 to distinguish between malignant and benign ovarian tumors.
KW - CARCINOMA
KW - EXPRESSION
KW - IMMUNITY
KW - IL-7
U2 - 10.1158/1078-0432.CCR-06-1828
DO - 10.1158/1078-0432.CCR-06-1828
M3 - Article
VL - 13
SP - 2385
EP - 2391
JO - Clinical Cancer Research
JF - Clinical Cancer Research
SN - 1078-0432
IS - 8
ER -