Shifts in the Microbial Population in Relation to in situ Caries Progression

R. Z. Thomas*, V. Zijnge, A. Cicek, J. J. de Soet, H. J. M. Harmsen, M. C. D. N. J. M. Huysmans

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites. Copyright (c) 2012 S. Karger AG, Basel

Original languageEnglish
Pages (from-to)427-431
Number of pages5
JournalCARIES RESEARCH
Volume46
Issue number5
DOIs
Publication statusPublished - 2012

Keywords

  • Denaturing gradient gel electrophoresis
  • Dental plaque
  • In situ caries progression
  • Microbial populations
  • GRADIENT GEL-ELECTROPHORESIS
  • SECONDARY CARIES
  • DENTAL-CARIES
  • MICROFLORA
  • PLAQUE
  • ADULTS

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