Shifts in the Microbial Population in Relation to in situ Caries Progression

R. Z. Thomas; V. Zijnge; A. Cicek; J. J. de Soet; H. J. M. Harmsen; M. C. D. N. J. M. Huysmans

doi:10.1159/000339482

Shifts in the Microbial Population in Relation to in situ Caries Progression

R. Z. Thomas^*, V. Zijnge, A. Cicek, J. J. de Soet, H. J. M. Harmsen, M. C. D. N. J. M. Huysmans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites. Copyright (c) 2012 S. Karger AG, Basel

Original language	English
Pages (from-to)	427-431
Number of pages	5
Journal	Caries Research
Volume	46
Issue number	5
DOIs	https://doi.org/10.1159/000339482
Publication status	Published - 2012

Keywords

Denaturing gradient gel electrophoresis
Dental plaque
In situ caries progression
Microbial populations
GRADIENT GEL-ELECTROPHORESIS
SECONDARY CARIES
DENTAL-CARIES
MICROFLORA
PLAQUE
ADULTS

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Cite this

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title = "Shifts in the Microbial Population in Relation to in situ Caries Progression",

abstract = "The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites. Copyright (c) 2012 S. Karger AG, Basel",

keywords = "Denaturing gradient gel electrophoresis, Dental plaque, In situ caries progression, Microbial populations, GRADIENT GEL-ELECTROPHORESIS, SECONDARY CARIES, DENTAL-CARIES, MICROFLORA, PLAQUE, ADULTS",

author = "Thomas, {R. Z.} and V. Zijnge and A. Cicek and {de Soet}, {J. J.} and Harmsen, {H. J. M.} and Huysmans, {M. C. D. N. J. M.}",

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T1 - Shifts in the Microbial Population in Relation to in situ Caries Progression

AU - Thomas, R. Z.

AU - Zijnge, V.

AU - Cicek, A.

AU - de Soet, J. J.

AU - Harmsen, H. J. M.

AU - Huysmans, M. C. D. N. J. M.

PY - 2012

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N2 - The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites. Copyright (c) 2012 S. Karger AG, Basel

AB - The shift in microbial diversity from young to mature plaque, related to caries activity on sound and restored surfaces, was studied using denaturing gradient gel electrophoresis. During a 20-week in situ study on caries progression 8 subjects wearing restored and unrestored dentin and enamel sections, biofilm was sampled after 1 and 20 weeks (young or mature plaque). A higher microbial diversity (mature plaque) was seen in caries-active compared to caries-free subjects. Rothia dentocariosa and Scardovia inopinata were absent from all caries-free sites, but appeared in 50% of the caries-active sites. Copyright (c) 2012 S. Karger AG, Basel

KW - Denaturing gradient gel electrophoresis

KW - Dental plaque

KW - In situ caries progression

KW - Microbial populations

KW - GRADIENT GEL-ELECTROPHORESIS

KW - SECONDARY CARIES

KW - DENTAL-CARIES

KW - MICROFLORA

KW - PLAQUE

KW - ADULTS

U2 - 10.1159/000339482

DO - 10.1159/000339482

M3 - Article

SN - 0008-6568

VL - 46

SP - 427

EP - 431

JO - Caries Research

JF - Caries Research

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ER -