Short-term antioxidative responses of 15 microalgae exposed to excessive irradiance including ultraviolet radiation

Paul J. Janknegt*, C. Marco De Graaff, Willem H. Van De Poll, Ronald J. W. Visser, Jan W. Rijstenbil, Anita G. J. Buma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

Short-term photosensitivity and oxidative stress responses were compared for three groups of marine microalgae: Antarctic microalgae, temperate diatoms and temperate flagellates. In total, 15 low-light-acclimated species were exposed to simulated surface irradiance including ultraviolet radiation (SSI). Photosensitivity was assessed as the rate of recovery of Fv/Fm in the hours following SSI treatment. Before, during and after the SSI treatment, oxidative stress responses were assessed by following xanthophyll content and cycling, and activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase, and glutathione redox status. When acclimated to low irradiance, antioxidant levels were not group specific. Superoxide dismutase activity was positively correlated with cell size, whereas in general, ascorbate peroxidase activity appeared to be lower and glutathione redox status appeared to be higher in the Antarctic than in the temperate species. After SSI exposure, the strong inhibition of PSII was followed by variable rates of recovery, although four species remained photosynthetically inactive. SSI tolerance appeared unrelated to geographic or taxonomic background, or to cell size. PSII recovery was enhanced in species with decreasing superoxide dismutase activity, glutathione redox status and increased xanthophyll cycle activity. We conclude that antioxidant responses are highly species specific and not related to the geographic or taxonomic background. Furthermore, xanthophyll cycling seems more important than antioxidants. Finally, it can be hypothesized that glutathione could function as a stress sensor and response regulator.

Original languageEnglish
Pages (from-to)525-539
Number of pages15
JournalEuropean Journal of Phycology
Volume44
Issue number4
DOIs
Publication statusPublished - 2009

Keywords

  • ascorbate peroxidase
  • glutathione
  • glutathione reductase
  • microalgae
  • photo-induced oxidative stress
  • photosensitivity
  • photosystem II efficiency
  • pigments
  • superoxide dismutase
  • xanthophyll cycle
  • UV-B RADIATION
  • NATURAL PHYTOPLANKTON COMMUNITIES
  • ASCORBATE PEROXIDASE ISOENZYMES
  • CHAETOCEROS-BREVIS BACILLARIOPHYCEAE
  • DIATOM PHAEODACTYLUM-TRICORNUTUM
  • OXIDATIVE STRESS TOLERANCE
  • PROGRAMMED CELL-DEATH
  • SUPEROXIDE-DISMUTASE
  • PHOTOSYNTHETIC ORGANISMS
  • DIADINOXANTHIN CYCLE

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