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Abstract

Aim: To develop a simple and robust LC-MS/MS method to quantify concentrations of micafungin in human plasma for pharmacokinetic studies and therapeutic drug monitoring. Methods: Sample preparation involved protein precipitation with acetonitrile: methanol (83: 17% v/v) and [C-13(6)]-micafungin as internal standard. A rapid and selective method for micafungin was validated across a range of 0.200-10.0 mg/l. Results: The calculated accuracy for the eight-point calibration ranged from 0.7 to 5.3%. Within-run precision ranged from 0.8 to 5.9%, between-run precision ranged from 0.7 to 3.1%, and overall precision ranged from 1.3 to 6.6%. Conclusion: A simple and robust LC-MS/MS method for analyzing micafungin in human plasma has been validated and was utilized for quantification of micafungin.

Original languageEnglish
Pages (from-to)877-886
Number of pages10
JournalBioanalysis
Volume10
Issue number11
Early online date4-Jun-2018
DOIs
Publication statusPublished - Jun-2018

Keywords

  • isotopically labeled internal standard
  • LC-MS/MS
  • micafungin
  • therapeutic drug monitoring
  • TANDEM MASS-SPECTROMETRY
  • MURINE CANDIDIASIS MODEL
  • PHARMACOKINETICS
  • PLASMA
  • MANAGEMENT
  • GUIDELINE
  • STABILITY
  • BLOOD

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