Simultaneous measurement of metabolic activity and membrane integrity in marine bacterioplankton determined by confocal laser-scanning microscopy

H Pirker, C Pausz, KE Stoderegger, GJ Herndl*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)
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Abstract

We simultaneously assessed the metabolic activity and viability of individual bacterioplankton cells in the coastal and open North Sea. Three different techniques were applied to determine cell features related to the physiological status of the cell. SYBR Green I was used to estimate the nucleic acid content of the cell. Propidium iodide (PI) stains cells with a compromised cell membrane, commonly interpreted as indicative of dead cells. Microautoradiography (MA) with radiolabeled glucose and leucine was applied to indicate metabolically active cells. The relative abundance of metabolically active cells determined by MA was usually

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalAquatic Microbial Ecology
Volume39
Issue number3
DOIs
Publication statusPublished - 20-Jun-2005
Externally publishedYes

Keywords

  • bacteria
  • propidium iodide method
  • microautoradiography
  • active versus dead
  • metabolic activity
  • single cell analysis
  • FLOW-CYTOMETRY
  • ESCHERICHIA-COLI
  • PROPIDIUM IODIDE
  • LARGE FRACTION
  • FRESH-WATER
  • BACTERIA
  • PROBES
  • CELLS
  • CTC
  • AUTORADIOGRAPHY

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