@article{44a2b177da91460b9d91eff8a8c7af57,
title = "Single cell genomes of Prochlorococcus, Synechococcus, and sympatric microbes from diverse marine environments",
abstract = "Prochlorococcus and Synechococcus are the dominant primary producers in marine ecosystems and perform a significant fraction of ocean carbon fixation. These cyanobacteria interact with a diverse microbial community that coexists with them. Comparative genomics of cultivated isolates has helped address questions regarding patterns of evolution and diversity among microbes, but the fraction that can be cultivated is miniscule compared to the diversity in the wild. To further probe the diversity of these groups and extend the utility of reference sequence databases, we report a data set of single cell genomes for 489 Prochlorococcus, 50 Synechococcus, 9 extracellular virus particles, and 190 additional microorganisms from a diverse range of bacterial, archaeal, and viral groups. Many of these uncultivated single cell genomes are derived from samples obtained on GEOTRACES cruises and at well-studied oceanographic stations, each with extensive suites of physical, chemical, and biological measurements. The genomic data reported here greatly increases the number of available Prochlorococcus genomes and will facilitate studies on evolutionary biology, microbial ecology, and biological oceanography.",
author = "Berube, \{Paul M.\} and Biller, \{Steven J.\} and Thomas Hackl and Hogle, \{Shane L.\} and Satinsky, \{Brandon M.\} and Becker, \{Jamie W.\} and Rogier Braakman and Collins, \{Sara B.\} and Libusha Kelly and Jessie Berta-Thompson and Allison Coe and Kristin Bergauer and Bouman, \{Heather A.\} and Browning, \{Thomas J.\} and \{De Corte\}, Daniele and Christel Hassler and Yotam Hulata and Jacquot, \{Jeremy E.\} and Maas, \{Elizabeth W.\} and Thomas Reinthaler and Eva Sintes and Taichi Yokokawa and Debbie Lindell and Ramunas Stepanauskas and Chisholm, \{Sallie W.\}",
note = "Funding Information: This work was supported in part by grants from the Simons Foundation (Life Sciences Project Award ID 337262, S.W.C.; SCOPE Award ID 329108, S.W.C.; Life Sciences Project Award ID 426570SP, E.V.A.), the Moore Foundation (Grant IDs GBMF495 and GBMF4511), and the National Science Foundation (OCE-1153588, OCE-1356460, and DBI-0424599 to S.W.C. and OCE-1335810 to R.S.). We are grateful for the support of chief scientists: E. Virginia Armbrust (University of Washington), Andrew Bowie (University of Tasmania), Philip Boyd (University of Tasmania), Edward Boyle (Massachusetts Institute of Technology), Gregory Cutter (Old Dominion University), Loes Gerringa (NIOZ Royal Netherlands Institute for Sea Research), Gideon Henderson (University of Oxford), William Jenkins (Woods Hole Oceanographic Institution), Daniel Repeta (Woods Hole Oceanographic Institution), and Micha Rijkenberg (NIOZ Royal Netherlands Institute for Sea Research), as well as Gerhard Herndl (University of Vienna), James Moffett (University of Southern California), and Hein de Baar (NIOZ Royal Netherlands Institute for Sea Research) for organizational and logistical support of bioGEOTRACES. We thank the HOT, BATS, and SCOPE cruise operations teams and Debbie A. Hulston (NIWA) for assistance with sampling, as well as the captains and crew of the R/V Kilo Moana, R/V Ka`imikai-O-Kanaloa, R/V Atlantic Explorer, R/V Pelagia, R/V Southern Surveyor, R/V Tangaroa, RRS James Cook, RRS Discovery, R/V Melville, and R/V Knorr. We also thank the staff of the Bigelow Laboratory for Ocean Sciences{\textquoteright} Single Cell Genomics Center for the generation of single cell genomics data. This paper is a contribution from the Simons Collaboration on Ocean Processes and Ecology (SCOPE) and from the NSF Center for Microbial Oceanography: Research and Education (C-MORE). Publisher Copyright: {\textcopyright} The Author(s) 2018.",
year = "2018",
doi = "10.1038/sdata.2018.154",
language = "English",
volume = "5",
journal = "Scientific Data",
issn = "2052-4463",
publisher = "Nature Publishing Group",
}