Single-cell sequencing to quantify genomic integrity in cancer

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Abstract

The use of single-cell DNA sequencing (sc-seq) techniques for the diagnosis, prognosis and treatment of cancer is a rapidly developing field. Sc-seq research is gaining momentum by decreased sequencing costs and continuous improvements in techniques. In this review, we provide an overview of recent advancements in the field of sc-seq in cancer and we discuss how sc-seq can contribute to improved care for cancer patients. Sc-seq has made it possible to study the genomes of individual cancer cells from primary tumors, metastases and circulating tumor cells, revealing inter- and intra-tumor heterogeneity, which cannot be detected using other methods. We review studies on individual human cancer cells in relation to prognosis and treatment response. Finally, future perspectives of sc-seq in cancer diagnosis and treatment are discussed with a focus on the use of circulating tumor cells to monitor therapy response and the development of personalized treatments based on knowledge about the genomic heterogeneity.

Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalInternational journal of biochemistry & cell biology
Volume94
Early online date23-Sep-2017
DOIs
Publication statusPublished - Jan-2018

Keywords

  • Single-cell sequencing
  • Intra-tumor heterogeneity
  • Tumor evolution
  • Circulating tumor cells
  • CIRCULATING TUMOR-CELLS
  • BREAST-CANCER
  • LUNG-CANCER
  • EVOLUTION
  • NUCLEOTIDE
  • ANEUPLOIDY
  • ORIGIN

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