Single guard cell recordings in intact plants: light-induced hyperpolarization of the plasma membrane

MRG Roelfsema, R Steinmeyer, M Staal, R Hedrich*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    74 Citations (Scopus)

    Abstract

    Guard cells are electrically isolated from other plant cells and therefore offer the unique possibility to conduct current- and voltage-clamp recordings on single cells in an intact plant. Guard cells in their natural environment were impaled with double-barreled electrodes and found to exhibit three physiological states. A minority of cells were classified as far-depolarized cells. These cells exhibited positive membrane potentials and were dominated by the activity of voltage-dependent anion channels. All other cells displayed both outward and inward rectifying K+-channel activity. These cells were either depolarized or hyperpolarized, with average membrane potentials of -41 mV (SD 16) and -112 mV (SD 19), respectively. Depolarized guard cells extrude K+ through outward rectifying channels, while K+ is taken up via inward rectifying channels in hyperpolarized cells. Upon a light/dark transition, guard cells that were hyperpolarized in the light switched to the depolarized state. The depolarization was accompanied by a 35 pA decrease in pump current and an increase in the conductance of inward rectifying channels. Both an increase in pump current and a decrease in the conductance of the inward rectifier were triggered by blue light, while red light was ineffective. From these studies we conclude that light modulates plasma membrane transport through large membrane potential changes, reversing the K+-efflux via outward rectifying channels to a K+-influx via inward rectifying channels.

    Original languageEnglish
    Pages (from-to)1-13
    Number of pages13
    JournalPlant Journal
    Volume26
    Issue number1
    Publication statusPublished - Apr-2001

    Keywords

    • guard cell
    • intact plant
    • light-induced
    • membrane potential
    • inward rectifying K+-channel
    • DEPENDENT ANION CHANNELS
    • BLUE-LIGHT
    • K+ CHANNELS
    • VICIA-FABA
    • ABSCISIC-ACID
    • ION CHANNELS
    • APOPLASTIC ACIDIFICATION
    • SIGNAL-TRANSDUCTION
    • OUTWARD RECTIFIER
    • STOMATAL MOVEMENT

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