Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs

Valeria Kalienkova, Carolina Alvadia, Vanessa Clerico Mosina, Cristina Paulino*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

4 Citations (Scopus)

Abstract

Single-particle cryo-electron microscopy has become an indispensable technique in structural biology. In particular when studying membrane proteins, it allows the use of membrane-mimicking tools, which can be crucial for a comprehensive understanding of the structure-function relationship of the protein in its native environment. In this chapter we focus on the application of nanodiscs and use our recent studies on the TMEM16 family as an example.

Original languageEnglish
Title of host publicationExpression, Purification, and Structural Biology of Membrane Proteins
EditorsCamilo Perez, Timm Maier
Place of Publication New York
PublisherHumana, New York, NY
Pages245-273
Number of pages29
ISBN (Electronic)978-1-0716-0373-4
ISBN (Print)978-1-0716-0372-7
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2127
ISSN (Print)1064-3745

Keywords

  • Cryo-EM
  • Single particle analysis
  • nanodiscs
  • membrane proteins
  • structural biology

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