Smart probe for simultaneous detection of copper ion, pyrophosphate, and alkaline phosphatase in vitro and in clinical samples

Sonia Kiran, Renuka Khatik, Romana Schirhagl*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)
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Abstract

Wilson's disease (WD), which might lead to acute liver failure, is an inherited disorder characterized by accumulation of copper (Cu2+) in the brain, the liver, and other vital organs. In the clinic, decreased serum alkaline phosphatase (ALP) concentration is used for WD diagnosis. But to the best of our knowledge, using a fluorescent probe to simultaneously detect multiple factors in WD (e.g., Cu2+, pyrophosphate (PPi), and ALP) has not been reported. Herein, we rationally designed a fluorescent switch (E)-8-((4-methylbenzylidene)amino)napthalen-1-amine (L) and successfully applied it for sequential and selective detections of Cu2+, PPi, and ALP in vitro, in living cells and synovial fluid samples with "Off," "On," and "Off" fluorescence signals, respectively. Considering the obvious correlations among Cu2+, PPi, and ALP in WD, we envision that our fluorescent probe L could be applied to in vitro diagnosing WD in the near future.

Original languageEnglish
Pages (from-to)6475-6485
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number24
DOIs
Publication statusPublished - Sep-2019

Keywords

  • Wilson's disease
  • Copper ion
  • Pyrophosphate
  • Alkaline phosphatase
  • Fluorescent probe
  • SELECTIVE FLUORESCENT CHEMOSENSOR
  • DOPED CARBON DOTS
  • ON-OFF ASSAY
  • SENSITIVE DETECTION
  • AQUEOUS-MEDIUM
  • SENSOR
  • CU2+
  • RECOGNITION
  • SWITCH
  • MECHANISMS

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