Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study

Therese S. Lapperre; Jacob K. Sont; Annemarie van Schadewijk; Margot Gosman; Ingeborg M. Bajema; Wim Timens; Thais Mauad; Pieter S. Hiemstra; H. F. Kauffman; H. M. Boezen; D. F. Jansen; J. Vonk; M. D. W. Barentsen; M. Zeinstra-Smit; A. J. Luteijn; T. van der Molen; G. ter Veen; N. H. T. ten Hacken; H. A. M. Kerstjens; M. S. van Maaren; D. S. Postma; C. A. Veltman; A. Verbokkem; I. Verhage; H. K. Vink-Kloosters; J. B. Snoeck-Stroband; H. Thiadens; J. K. Sont; I. Bajema; J. Gast-Strookman; P. S. Hiemstra; K. Janssen; T. S. Lapperre; K. F. Rabe; A. van Schadewijk; J. A. Schrumpf; J. Smit-Bakker; J. Stolk; A. C. J. A. Tire; H. van der Veen; M. M. E. Wijffels; L. N. A. Willems; P. J. Sterk; T. Mauad; GLUCOLD Study Grp; D.  de Reus

doi:10.1186/1465-9921-8-85

Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study

Therese S. Lapperre^*, Jacob K. Sont, Annemarie van Schadewijk, Margot Gosman, Ingeborg M. Bajema, Wim Timens, Thais Mauad, Pieter S. Hiemstra, H. F. Kauffman, H. M. Boezen, D. F. Jansen, J. Vonk, M. D. W. Barentsen, M. Zeinstra-Smit, A. J. Luteijn, T. van der Molen, G. ter Veen, N. H. T. ten Hacken, H. A. M. Kerstjens, M. S. van MaarenD. S. Postma, C. A. Veltman, A. Verbokkem, I. Verhage, H. K. Vink-Kloosters, J. B. Snoeck-Stroband, H. Thiadens, J. K. Sont, I. Bajema, J. Gast-Strookman, P. S. Hiemstra, K. Janssen, T. S. Lapperre, K. F. Rabe, A. van Schadewijk, J. A. Schrumpf, J. Smit-Bakker, J. Stolk, A. C. J. A. Tire, H. van der Veen, M. M. E. Wijffels, L. N. A. Willems, P. J. Sterk, T. Mauad, GLUCOLD Study Grp, D. de Reus

^*Corresponding author for this work

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Abstract

Background: Chronic Obstructive Pulmonary Disease ( COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor ( EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation.

Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 +/- 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers ( n = 42, median cessation duration 3.5 years), postbronchodilator FEV 63 +/- 9% predicted. Squamous cell metaplasia (%), goblet cell ( PAS/Alcian Blue(+)) area (%), proliferating (Ki- 67(+)) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies.

Results: Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD ( p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (

Conclusion: Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).

Original language	English
Article number	85
Number of pages	9
Journal	Respiratory Research
Volume	8
DOIs	https://doi.org/10.1186/1465-9921-8-85
Publication status	Published - 26-Nov-2007

Keywords

OBSTRUCTIVE PULMONARY-DISEASE
GROWTH-FACTOR RECEPTOR
CHRONIC MUCUS HYPERSECRETION
HUMAN NEUTROPHIL DEFENSINS
AIR-FLOW OBSTRUCTION
CURRENT SMOKERS
LUNG HEALTH
PERIPHERAL AIRWAYS
GOBLET CELL
EX-SMOKERS

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Lapperre, T. S., Sont, J. K., van Schadewijk, A., Gosman, M., Bajema, I. M., Timens, W., Mauad, T., Hiemstra, P. S., Kauffman, H. F., Boezen, H. M., Jansen, D. F., Vonk, J., Barentsen, M. D. W., Zeinstra-Smit, M., Luteijn, A. J., van der Molen, T., ter Veen, G., ten Hacken, N. H. T., Kerstjens, H. A. M., ... de Reus, D. (2007). Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study. Respiratory Research, 8, [85]. https://doi.org/10.1186/1465-9921-8-85

Lapperre, Therese S. ; Sont, Jacob K. ; van Schadewijk, Annemarie ; Gosman, Margot ; Bajema, Ingeborg M. ; Timens, Wim ; Mauad, Thais ; Hiemstra, Pieter S. ; Kauffman, H. F. ; Boezen, H. M. ; Jansen, D. F. ; Vonk, J. ; Barentsen, M. D. W. ; Zeinstra-Smit, M. ; Luteijn, A. J. ; van der Molen, T. ; ter Veen, G. ; ten Hacken, N. H. T. ; Kerstjens, H. A. M. ; van Maaren, M. S. ; Postma, D. S. ; Veltman, C. A. ; Verbokkem, A. ; Verhage, I. ; Vink-Kloosters, H. K. ; Snoeck-Stroband, J. B. ; Thiadens, H. ; Sont, J. K. ; Bajema, I. ; Gast-Strookman, J. ; Hiemstra, P. S. ; Janssen, K. ; Lapperre, T. S. ; Rabe, K. F. ; van Schadewijk, A. ; Schrumpf, J. A. ; Smit-Bakker, J. ; Stolk, J. ; Tire, A. C. J. A. ; van der Veen, H. ; Wijffels, M. M. E. ; Willems, L. N. A. ; Sterk, P. J. ; Mauad, T. ; GLUCOLD Study Grp ; de Reus, D. . / Smoking cessation and bronchial epithelial remodelling in COPD : a cross-sectional study. In: Respiratory Research. 2007 ; Vol. 8.

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title = "Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study",

abstract = "Background: Chronic Obstructive Pulmonary Disease ( COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor ( EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation.Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 +/- 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers ( n = 42, median cessation duration 3.5 years), postbronchodilator FEV 63 +/- 9% predicted. Squamous cell metaplasia (%), goblet cell ( PAS/Alcian Blue(+)) area (%), proliferating (Ki- 67(+)) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies.Results: Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD ( p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (Conclusion: Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).",

keywords = "OBSTRUCTIVE PULMONARY-DISEASE, GROWTH-FACTOR RECEPTOR, CHRONIC MUCUS HYPERSECRETION, HUMAN NEUTROPHIL DEFENSINS, AIR-FLOW OBSTRUCTION, CURRENT SMOKERS, LUNG HEALTH, PERIPHERAL AIRWAYS, GOBLET CELL, EX-SMOKERS",

author = "Lapperre, {Therese S.} and Sont, {Jacob K.} and {van Schadewijk}, Annemarie and Margot Gosman and Bajema, {Ingeborg M.} and Wim Timens and Thais Mauad and Hiemstra, {Pieter S.} and Kauffman, {H. F.} and Boezen, {H. M.} and Jansen, {D. F.} and J. Vonk and Barentsen, {M. D. W.} and M. Zeinstra-Smit and Luteijn, {A. J.} and {van der Molen}, T. and {ter Veen}, G. and {ten Hacken}, {N. H. T.} and Kerstjens, {H. A. M.} and {van Maaren}, {M. S.} and Postma, {D. S.} and Veltman, {C. A.} and A. Verbokkem and I. Verhage and Vink-Kloosters, {H. K.} and Snoeck-Stroband, {J. B.} and H. Thiadens and Sont, {J. K.} and I. Bajema and J. Gast-Strookman and Hiemstra, {P. S.} and K. Janssen and Lapperre, {T. S.} and Rabe, {K. F.} and {van Schadewijk}, A. and Schrumpf, {J. A.} and J. Smit-Bakker and J. Stolk and Tire, {A. C. J. A.} and {van der Veen}, H. and Wijffels, {M. M. E.} and Willems, {L. N. A.} and Sterk, {P. J.} and T. Mauad and {GLUCOLD Study Grp} and {de Reus}, D.",

year = "2007",

month = nov,

day = "26",

doi = "10.1186/1465-9921-8-85",

language = "English",

volume = "8",

journal = "Respiratory Research",

issn = "1465-9921",

publisher = "BioMed Central Ltd.",

}

Lapperre, TS, Sont, JK, van Schadewijk, A, Gosman, M, Bajema, IM, Timens, W, Mauad, T, Hiemstra, PS, Kauffman, HF , Boezen, HM , Jansen, DF , Vonk, J, Barentsen, MDW, Zeinstra-Smit, M, Luteijn, AJ , van der Molen, T, ter Veen, G, ten Hacken, NHT, Kerstjens, HAM, van Maaren, MS, Postma, DS, Veltman, CA, Verbokkem, A, Verhage, I, Vink-Kloosters, HK, Snoeck-Stroband, JB, Thiadens, H, Sont, JK, Bajema, I, Gast-Strookman, J, Hiemstra, PS, Janssen, K, Lapperre, TS, Rabe, KF, van Schadewijk, A, Schrumpf, JA, Smit-Bakker, J, Stolk, J, Tire, ACJA, van der Veen, H, Wijffels, MME, Willems, LNA, Sterk, PJ, Mauad, T, GLUCOLD Study Grp & de Reus, D 2007, 'Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study', Respiratory Research, vol. 8, 85. https://doi.org/10.1186/1465-9921-8-85

Smoking cessation and bronchial epithelial remodelling in COPD : a cross-sectional study. / Lapperre, Therese S.; Sont, Jacob K.; van Schadewijk, Annemarie; Gosman, Margot; Bajema, Ingeborg M.; Timens, Wim; Mauad, Thais; Hiemstra, Pieter S.; Kauffman, H. F.; Boezen, H. M.; Jansen, D. F.; Vonk, J.; Barentsen, M. D. W.; Zeinstra-Smit, M.; Luteijn, A. J.; van der Molen, T.; ter Veen, G.; ten Hacken, N. H. T.; Kerstjens, H. A. M.; van Maaren, M. S.; Postma, D. S.; Veltman, C. A.; Verbokkem, A.; Verhage, I.; Vink-Kloosters, H. K.; Snoeck-Stroband, J. B.; Thiadens, H.; Sont, J. K.; Bajema, I.; Gast-Strookman, J.; Hiemstra, P. S.; Janssen, K.; Lapperre, T. S.; Rabe, K. F.; van Schadewijk, A.; Schrumpf, J. A.; Smit-Bakker, J.; Stolk, J.; Tire, A. C. J. A.; van der Veen, H.; Wijffels, M. M. E.; Willems, L. N. A.; Sterk, P. J.; Mauad, T.; GLUCOLD Study Grp; de Reus, D. .

In: Respiratory Research, Vol. 8, 85, 26.11.2007.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Smoking cessation and bronchial epithelial remodelling in COPD

T2 - a cross-sectional study

AU - Lapperre, Therese S.

AU - Sont, Jacob K.

AU - van Schadewijk, Annemarie

AU - Gosman, Margot

AU - Bajema, Ingeborg M.

AU - Timens, Wim

AU - Mauad, Thais

AU - Hiemstra, Pieter S.

AU - Kauffman, H. F.

AU - Boezen, H. M.

AU - Jansen, D. F.

AU - Vonk, J.

AU - Barentsen, M. D. W.

AU - Zeinstra-Smit, M.

AU - Luteijn, A. J.

AU - van der Molen, T.

AU - ter Veen, G.

AU - ten Hacken, N. H. T.

AU - Kerstjens, H. A. M.

AU - van Maaren, M. S.

AU - Postma, D. S.

AU - Veltman, C. A.

AU - Verbokkem, A.

AU - Verhage, I.

AU - Vink-Kloosters, H. K.

AU - Snoeck-Stroband, J. B.

AU - Thiadens, H.

AU - Sont, J. K.

AU - Bajema, I.

AU - Gast-Strookman, J.

AU - Hiemstra, P. S.

AU - Janssen, K.

AU - Lapperre, T. S.

AU - Rabe, K. F.

AU - van Schadewijk, A.

AU - Schrumpf, J. A.

AU - Smit-Bakker, J.

AU - Stolk, J.

AU - Tire, A. C. J. A.

AU - van der Veen, H.

AU - Wijffels, M. M. E.

AU - Willems, L. N. A.

AU - Sterk, P. J.

AU - Mauad, T.

AU - GLUCOLD Study Grp

AU - de Reus, D.

PY - 2007/11/26

Y1 - 2007/11/26

N2 - Background: Chronic Obstructive Pulmonary Disease ( COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor ( EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation.Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 +/- 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers ( n = 42, median cessation duration 3.5 years), postbronchodilator FEV 63 +/- 9% predicted. Squamous cell metaplasia (%), goblet cell ( PAS/Alcian Blue(+)) area (%), proliferating (Ki- 67(+)) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies.Results: Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD ( p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (Conclusion: Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).

AB - Background: Chronic Obstructive Pulmonary Disease ( COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor ( EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation.Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 +/- 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers ( n = 42, median cessation duration 3.5 years), postbronchodilator FEV 63 +/- 9% predicted. Squamous cell metaplasia (%), goblet cell ( PAS/Alcian Blue(+)) area (%), proliferating (Ki- 67(+)) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies.Results: Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD ( p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (Conclusion: Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years).

KW - OBSTRUCTIVE PULMONARY-DISEASE

KW - GROWTH-FACTOR RECEPTOR

KW - CHRONIC MUCUS HYPERSECRETION

KW - HUMAN NEUTROPHIL DEFENSINS

KW - AIR-FLOW OBSTRUCTION

KW - CURRENT SMOKERS

KW - LUNG HEALTH

KW - PERIPHERAL AIRWAYS

KW - GOBLET CELL

KW - EX-SMOKERS

U2 - 10.1186/1465-9921-8-85

DO - 10.1186/1465-9921-8-85

M3 - Article

C2 - 18039368

VL - 8

JO - Respiratory Research

JF - Respiratory Research

SN - 1465-9921

M1 - 85

ER -