Sol-gel transition in solutions of FG-Nups of the nuclear pore complex

Ali Ghavami, Erik Van der Giessen, Patrick R. Onck*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The nuclear pore complex (NPC) is a giant molecular gate that regulates the transport of molecules and macromolecules across the nuclear envelop in a selective and directional manner. Using a coarse-grained one-bead-per-amino-acid model and percolation analysis we have characterized the gel formation of solutions of FG-Nups that line the central channel of the NPC. We show that FG-Nup solutions can indeed form a percolated network (gel) when the concentration of the Nups exceeds a certain limit. Furthermore, we show that this critical concentration depends on the amino acid composition of the FG-Nups and correlates with the ratio between charged and hydrophobic amino acids. Comparison with the protein concentrations inside the yeast NPC reveals that it is very likely that the FG-Nups form a gel phase. (c) 2018 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalExtreme Mechanics Letters
Volume22
DOIs
Publication statusPublished - Jul-2018

Keywords

  • SINGLE-MOLECULE FLUORESCENCE
  • PERMEABILITY
  • NUCLEOPORINS
  • PROTEINS
  • TRANSPORT
  • CONFORMATIONS
  • SELECTIVITY
  • HYDROGELS
  • MEMBRANE
  • POLYMERS

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