Solubility of (1 leads to 3)-beta-D/(1 leads to 6)-beta-D-glucan in fungal walls: importance of presumed linkage between glucan and chitin.

JH Sietsma*, JGH Wessels

*Corresponding author for this work

    Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

    77 Citations (Scopus)


    In Saccharomyces cerevisiae, Neurospora crassa, Aspergillus nidulans and Coprinus cinereus most of the alkali-insoluble (1 leads to 3)-beta-D/(1 leads to 6)-beta-D-glucan of the wall can be extracted with dimethyl sulphoxide. The same fraction, and in Saccharomyces cerevisiae a small additional fraction, can be extracted by a destructive procedure involving 40% NaOH at 100 degrees C. The small fraction of the glucan which resists this treatment becomes soluble after a subsequent treatment with HNO2 indicating that it is covalently linked to chitin in the wall. In contrast, in Schizophyllum commune and Agaricus bisporus, nearly all the (1 leads to 3)-beta-D/(1 leads to 6)-beta-D-glucan appears to be held insoluble by linkage to chitin.
    Original languageEnglish
    Pages (from-to)209-212
    Number of pages4
    JournalJournal of general microbiology
    Issue numberJUL
    Publication statusPublished - 1981

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