SPATIAL AND TEMPORAL ACCUMULATION OF MESSENGER-RNAS ENCODING 2 COMMON LIGNIN PEROXIDASES IN PHANEROCHAETE-CHRYSOSPORIUM

SM MOUKHA*, HAB WOSTEN, EJ MYLIUS, M ASTHER, JGH WESSELS

*Corresponding author for this work

    Research output: Contribution to journalComment/Letter to the editorAcademicpeer-review

    21 Citations (Scopus)

    Abstract

    Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.

    Original languageEnglish
    Pages (from-to)3672-3678
    Number of pages7
    JournalJournal of Bacteriology
    Volume175
    Issue number11
    Publication statusPublished - Jun-1993

    Keywords

    • WHITE ROT FUNGI
    • DEGRADING BASIDIOMYCETE
    • MANGANESE PEROXIDASE
    • SCHIZOPHYLLUM-COMMUNE
    • WOOD
    • LOCALIZATION
    • DEGRADATION
    • EXPRESSION
    • SECRETION
    • PROTEINS

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