Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/luc mice

Huawei Su, Gooitzen M. van Dam, Carlijn I. Buis, Dorien S. Visser, Jan Willem Hesselink, Theo A. Schuurs, Henri G. D. Leuvenink, Christopher H. Contag, Robert J. Porte*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Upregulation of heme oxygenase-1 (HO-1) has been proposed as a critical mechanism protecting against cellular stress during liver transplantation, providing a potential target for new therapeutic interventions. We investigated the feasibility of in vivo bioluminescence imaging (BLI) to noninvasively quantify the spatiotemporal expression of HO-1 after warm hepatic ischemia in living animals. Luciferase activity was measured by BLI as an index of HO-1 transcription in transgenic reporter mice (Ho1-luc) at standardized time points after 60 minutes of warm hepatic ischemia. HO-1 mRNA levels were measured in postischemic livers of mice sacrificed at the same time points in separate experiments. Bioluminescent signals from postischemic liver lobes were first detected at 3 hours after reperfusion. Peak levels were reached at 9 hours, after which bioluminescent activity declined and returned to baseline values at 48 hours after reperfusion. Upregulation of HO-1 as detected by in vivo BLI was preceded by increased HO-1 mRNA expression and confirmed by enhanced immunohistochemical staining of hepatocytes. In conclusion, this study shows that in vivo BLI allows a sensitive assessment of HO-1 expression after hepatic ischemia in living animals. The capability of whole-body temporal imaging

Original languageEnglish
Pages (from-to)1634-1639
Number of pages6
JournalLiver Transplantation
Volume12
Issue number11
DOIs
Publication statusPublished - Nov-2006

Keywords

  • GENE-EXPRESSION
  • LIVING ANIMALS
  • RAT-LIVER
  • TRANSPLANTATION
  • PATTERNS
  • SYSTEM

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