@inbook{aa573b4b296949dc92dc3cbc479a8a42,
title = "Standardized Protocol for the Preparation of Precision-Cut Kidney Slices: A Translational Model of Renal Fibrosis",
abstract = "Renal fibrosis is a hallmark of progressive renal diseases. To date, there is a lack of effective therapeutics for the treatment of renal fibrosis, in part due to the scarcity of clinically relevant translational disease models. Since the early 1920s, hand-cut tissue slices have been used as a means to better understand organ (patho)physiology in a variety of scientific fields. From that time, the equipment and methodology for the preparation of tissue slices has continuously improved, thereby expanding the applicability of the model. Nowadays, precision-cut kidney slices (PCKS) have been demonstrated to be an extremely valuable translation model for renal (patho)physiology, bridging the gap between preclinical and clinical research. A key feature of PCKS is that the slices contain all cell types and acellular components of the whole organ in the original configuration while preserving cell-cell and cell-matrix interactions. In this chapter, we describe how to prepare PCKS and how the model can be implemented in fibrosis research.",
keywords = "Ex vivo, Kidney, Krumdieck tissue slicer, Precision-cut kidney slices, Preclinical drug testing, Renal fibrosis, Translational research",
author = "Jensen, {Michael Schou} and Camilla Merrild and Rikke N{\o}rregaard and Peter Olinga and Mutsaers, {Henricus A.M.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3179-9_9",
language = "English",
isbn = "978-1-0716-3178-2",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "123--134",
editor = "Hewitson, {Tim D. } and Toussaint, {Nigel D. } and Smith, {Edward R. }",
booktitle = "Methods in Molecular Biology",
}