Staphylococcal chromosomal cassette mec type I, spa type, and expression of Pls are determinants of reduced cellular invasiveness of methicillin-resistant Staphylococcus aureus isolates

Cornelia Werbick, Karsten Becker, Alexander Mellmann, Katri M. Juuti, Christof von Eiff, Georg Peters, Pentti I. Kuusela, Alexander W. Friedrich, Bhanu Sinha*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

We have shown previously that pls, which codes for the surface protein Pls of methicillin-resistant Staphylococcus aureus (MRSA), reduces adhesion to immobilized fibronectin, fibrinogen, laminin, and immunoglobulin G as well as invasion of host cells. Here, we tested a collection of 66 clinical MRSA isolates--48 negative for pls/Pls (pls(-)/Pls(-)), 15 positive for pls/Pls (pls(+)/Pls(+)), and 3 harboring the pls gene but not expressing Pls (pls(+)/Pls(-))--for cellular invasiveness. Invasion of 293 cells by pls(+)/Pls(+) strains was lower than that by the pls(-)/Pls(-) strains (median [range], 36% [22%-70%] vs. 93% [25%-162%]). The 3 pls(+)/Pls(-) strains (median [range], 95% [63%-103%]) were as invasive as the pls(-)/Pls(-) strains. In addition, we identified a pls(+)/Pls(+) staphylococcal chromosomal cassette mec (SCCmec) IV strain. In conclusion, 3 properties--pls/Pls, SCCmec type, and spa type--strongly predicted the cellular invasiveness of MRSA strains, as indicated by good clustering. In contrast, the spa type-deduced multilocus sequence typing clonal complex (MLST-CC) was not able to predict the invasiveness of MRSA strains equally well. The underlying mechanism remains to be elucidated.

Original languageEnglish
Pages (from-to)1678-1685
Number of pages8
JournalThe Journal of Infectious Diseases
Volume195
Issue number11
DOIs
Publication statusPublished - 1-Jun-2007
Externally publishedYes
Event10th International Symposium on Staphylococci and Staphylococcal Diseases - Charleston, Seychelles
Duration: 23-Oct-200427-Oct-2004

Keywords

  • FIBRONECTIN-BINDING PROTEINS
  • A GENE POLYMORPHISM
  • SURFACE PROTEIN
  • ENDOTHELIAL-CELLS
  • EPITHELIAL-CELLS
  • PANDEMIC CLONES
  • INVASION
  • STRAINS
  • IDENTIFICATION
  • ADHERENCE

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