Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions

Pim de Haan; Maciej Grajewski; Daan Zillen; Elisabeth Verpoorte

Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions

Pim de Haan, Maciej Grajewski, Daan Zillen, Elisabeth Verpoorte

Research output: Contribution to conference › Abstract › Academic

Abstract

Enzymatic digestion is a key process in the gastrointestinal tract that we recreated in an earlier miniaturized system. In order to avoid the need to continuously supply digestive enzymes, this work studied the immobilization of digestive enzymes in a microsystem. Flow-through reactors were produced by stereolithography and the digestive enzyme, alpha-amylase, was immobilized onto the channel walls. The activity of immobilized alpha-amylase was determined by digestion of starch, with potential applications in organs-on-a-chip or other enzymatic conversions.

Original language	English
Pages	1169
Number of pages	2
Publication status	Published - 13-Oct-2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021): MicroTAS 2021 - Palm Springs, United States Duration: 10-Oct-2021 → 14-Oct-2021 Conference number: 25 http://microtas2021.org

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)
Abbreviated title	µTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/2021 → 14/10/2021
Internet address	http://microtas2021.org

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title = "Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions",

abstract = "Enzymatic digestion is a key process in the gastrointestinal tract that we recreated in an earlier miniaturized system. In order to avoid the need to continuously supply digestive enzymes, this work studied the immobilization of digestive enzymes in a microsystem. Flow-through reactors were produced by stereolithography and the digestive enzyme, alpha-amylase, was immobilized onto the channel walls. The activity of immobilized alpha-amylase was determined by digestion of starch, with potential applications in organs-on-a-chip or other enzymatic conversions. ",

author = "{de Haan}, Pim and Maciej Grajewski and Daan Zillen and Elisabeth Verpoorte",

year = "2021",

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Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions. / de Haan, Pim; Grajewski, Maciej; Zillen, Daan et al.
2021. 1169 Abstract from 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, California, United States.

Research output: Contribution to conference › Abstract › Academic

TY - CONF

T1 - Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions

AU - de Haan, Pim

AU - Grajewski, Maciej

AU - Zillen, Daan

AU - Verpoorte, Elisabeth

N1 - Conference code: 25

PY - 2021/10/13

Y1 - 2021/10/13

N2 - Enzymatic digestion is a key process in the gastrointestinal tract that we recreated in an earlier miniaturized system. In order to avoid the need to continuously supply digestive enzymes, this work studied the immobilization of digestive enzymes in a microsystem. Flow-through reactors were produced by stereolithography and the digestive enzyme, alpha-amylase, was immobilized onto the channel walls. The activity of immobilized alpha-amylase was determined by digestion of starch, with potential applications in organs-on-a-chip or other enzymatic conversions.

AB - Enzymatic digestion is a key process in the gastrointestinal tract that we recreated in an earlier miniaturized system. In order to avoid the need to continuously supply digestive enzymes, this work studied the immobilization of digestive enzymes in a microsystem. Flow-through reactors were produced by stereolithography and the digestive enzyme, alpha-amylase, was immobilized onto the channel walls. The activity of immobilized alpha-amylase was determined by digestion of starch, with potential applications in organs-on-a-chip or other enzymatic conversions.

M3 - Abstract

SP - 1169

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)

Y2 - 10 October 2021 through 14 October 2021

ER -

Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions

Abstract

Conference

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