Steric exclusion and protein conformation determine the localization of plasma membrane transporters

Frans Bianchi, Łukasz Syga, Gemma Moiset, Dian Spakman, Paul E Schavemaker, Christiaan M Punter, Anne-Bart Seinen, Antoine M van Oijen, Andrew Robinson, Bert Poolman

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)
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Abstract

The plasma membrane (PM) of Saccharomyces cerevisiae contains membrane compartments, MCC/eisosomes and MCPs, named after the protein residents Can1 and Pma1, respectively. Using high-resolution fluorescence microscopy techniques we show that Can1 and the homologous transporter Lyp1 are able to diffuse into the MCC/eisosomes, where a limited number of proteins are conditionally trapped at the (outer) edge of the compartment. Upon addition of substrate, the immobilized proteins diffuse away from the MCC/eisosomes, presumably after taking a different conformation in the substrate-bound state. Our data indicate that the mobile fraction of all integral plasma membrane proteins tested shows extremely slow Brownian diffusion through most of the PM. We also show that proteins with large cytoplasmic domains, such as Pma1 and synthetic chimera of Can1 and Lyp1, are excluded from the MCC/eisosomes. We hypothesize that the distinct localization patterns found for these integral membrane proteins in S. cerevisiae arises from a combination of slow lateral diffusion, steric exclusion, and conditional trapping in membrane compartments.

Original languageEnglish
Article number501
Number of pages13
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 5-Feb-2018

Keywords

  • plasma membrane
  • transporters
  • localization
  • MCC/eisosomes
  • MCPs
  • high-resolution microscopy
  • S. cerevisiae

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