Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Sander van Leeuwen; S. Kralj; G.H. van Geel-Schutten; Gerrit Gerwig; L. Dijkhuizen; J.P. Kamerling

doi:10.1016/j.carres.2008.01.042

Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Sander van Leeuwen, S. Kralj, G.H. van Geel-Schutten, Gerrit Gerwig, L. Dijkhuizen, J.P. Kamerling

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Abstract

The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1 -> 3, 1 -> 6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and ID/2D (1)H and (13)C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:

[GRAPHICS]

Original language	English
Pages (from-to)	1237-1250
Number of pages	14
Journal	Carbohydrate Research
Volume	343
Issue number	7
DOIs	https://doi.org/10.1016/j.carres.2008.01.042
Publication status	Published - 19-May-2008

Keywords

alpha-D-glucan
polysaccharide structural analysis
GTF180 glucansucrase
(1)H NMR spectroscopy
Lactobacillus reuteri 180
LACTIC-ACID BACTERIA
FORMS

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title = "Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme",

abstract = "The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1 -> 3, 1 -> 6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and ID/2D (1)H and (13)C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:[GRAPHICS](c) 2008 Elsevier Ltd. All rights reserved.",

keywords = "alpha-D-glucan, polysaccharide structural analysis, GTF180 glucansucrase, (1)H NMR spectroscopy, Lactobacillus reuteri 180, LACTIC-ACID BACTERIA, FORMS",

author = "{van Leeuwen}, Sander and S. Kralj and {van Geel-Schutten}, G.H. and Gerrit Gerwig and L. Dijkhuizen and J.P. Kamerling",

year = "2008",

month = may,

day = "19",

doi = "10.1016/j.carres.2008.01.042",

language = "English",

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T1 - Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

AU - van Leeuwen, Sander

AU - Kralj, S.

AU - van Geel-Schutten, G.H.

AU - Gerwig, Gerrit

AU - Dijkhuizen, L.

AU - Kamerling, J.P.

PY - 2008/5/19

Y1 - 2008/5/19

N2 - The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1 -> 3, 1 -> 6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and ID/2D (1)H and (13)C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:[GRAPHICS](c) 2008 Elsevier Ltd. All rights reserved.

AB - The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1 -> 3, 1 -> 6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and ID/2D (1)H and (13)C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:[GRAPHICS](c) 2008 Elsevier Ltd. All rights reserved.

KW - alpha-D-glucan

KW - polysaccharide structural analysis

KW - GTF180 glucansucrase

KW - (1)H NMR spectroscopy

KW - Lactobacillus reuteri 180

KW - LACTIC-ACID BACTERIA

KW - FORMS

U2 - 10.1016/j.carres.2008.01.042

DO - 10.1016/j.carres.2008.01.042

M3 - Article

SN - 1873-426X

VL - 343

SP - 1237

EP - 1250

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 7

ER -

Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Abstract

Keywords

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