Structural-Functional Role of Chloride in Photosystem II

Ivan Rivalta; Muhamed Amin; Sandra Luber; Serguei Vassiliev; Ravi Pokhrel; Yasufumi Umena; Keisuke Kawakami; Jian-Ren Shen; Nobuo Kamiya; Doug Bruce; Gary W. Brudvig; M. R. Gunner; Victor S. Batista

doi:10.1021/bi200685w

Structural-Functional Role of Chloride in Photosystem II

Ivan Rivalta^*, Muhamed Amin, Sandra Luber, Serguei Vassiliev, Ravi Pokhrel, Yasufumi Umena, Keisuke Kawakami, Jian-Ren Shen, Nobuo Kamiya, Doug Bruce, Gary W. Brudvig, M. R. Gunner, Victor S. Batista

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

120 Citations (Scopus)

Abstract

Chloride binding in photosystem II (PSII) is essential for photosynthetic water oxidation. However, the functional roles of chloride and possible binding sites, during oxygen evolution, remain controversial. This paper examines the functions of chloride based on its binding site revealed in the X-ray crystal structure of PSII at 1.9 angstrom resolution. We find that chloride depletion induces formation of a salt bridge between D2-K317 and D1-D61 that could suppress the transfer of protons to the lumen.

Original language	English
Pages (from-to)	6312-6315
Number of pages	4
Journal	Biochemistry
Volume	50
Issue number	29
DOIs	https://doi.org/10.1021/bi200685w
Publication status	Published - 26-Jul-2011
Externally published	Yes

Keywords

OXYGEN-EVOLVING COMPLEX
PHOTOSYNTHETIC WATER OXIDATION
X-RAY CRYSTALLOGRAPHY
O-2-EVOLVING COMPLEX
ANGSTROM RESOLUTION
MANGANESE CLUSTER
ELECTRON-TRANSFER
CRYSTAL-STRUCTURE
BINDING
MECHANISM

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Structural–Functional Role of Chloride in Photosystem II
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@article{4b9360c0f396458ea297823738a2d9ca,

title = "Structural-Functional Role of Chloride in Photosystem II",

abstract = "Chloride binding in photosystem II (PSII) is essential for photosynthetic water oxidation. However, the functional roles of chloride and possible binding sites, during oxygen evolution, remain controversial. This paper examines the functions of chloride based on its binding site revealed in the X-ray crystal structure of PSII at 1.9 angstrom resolution. We find that chloride depletion induces formation of a salt bridge between D2-K317 and D1-D61 that could suppress the transfer of protons to the lumen.",

keywords = "OXYGEN-EVOLVING COMPLEX, PHOTOSYNTHETIC WATER OXIDATION, X-RAY CRYSTALLOGRAPHY, O-2-EVOLVING COMPLEX, ANGSTROM RESOLUTION, MANGANESE CLUSTER, ELECTRON-TRANSFER, CRYSTAL-STRUCTURE, BINDING, MECHANISM",

author = "Ivan Rivalta and Muhamed Amin and Sandra Luber and Serguei Vassiliev and Ravi Pokhrel and Yasufumi Umena and Keisuke Kawakami and Jian-Ren Shen and Nobuo Kamiya and Doug Bruce and Brudvig, {Gary W.} and Gunner, {M. R.} and Batista, {Victor S.}",

year = "2011",

month = jul,

day = "26",

doi = "10.1021/bi200685w",

language = "English",

volume = "50",

pages = "6312--6315",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "AMER CHEMICAL SOC",

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TY - JOUR

T1 - Structural-Functional Role of Chloride in Photosystem II

AU - Rivalta, Ivan

AU - Amin, Muhamed

AU - Luber, Sandra

AU - Vassiliev, Serguei

AU - Pokhrel, Ravi

AU - Umena, Yasufumi

AU - Kawakami, Keisuke

AU - Shen, Jian-Ren

AU - Kamiya, Nobuo

AU - Bruce, Doug

AU - Brudvig, Gary W.

AU - Gunner, M. R.

AU - Batista, Victor S.

PY - 2011/7/26

Y1 - 2011/7/26

N2 - Chloride binding in photosystem II (PSII) is essential for photosynthetic water oxidation. However, the functional roles of chloride and possible binding sites, during oxygen evolution, remain controversial. This paper examines the functions of chloride based on its binding site revealed in the X-ray crystal structure of PSII at 1.9 angstrom resolution. We find that chloride depletion induces formation of a salt bridge between D2-K317 and D1-D61 that could suppress the transfer of protons to the lumen.

AB - Chloride binding in photosystem II (PSII) is essential for photosynthetic water oxidation. However, the functional roles of chloride and possible binding sites, during oxygen evolution, remain controversial. This paper examines the functions of chloride based on its binding site revealed in the X-ray crystal structure of PSII at 1.9 angstrom resolution. We find that chloride depletion induces formation of a salt bridge between D2-K317 and D1-D61 that could suppress the transfer of protons to the lumen.

KW - OXYGEN-EVOLVING COMPLEX

KW - PHOTOSYNTHETIC WATER OXIDATION

KW - X-RAY CRYSTALLOGRAPHY

KW - O-2-EVOLVING COMPLEX

KW - ANGSTROM RESOLUTION

KW - MANGANESE CLUSTER

KW - ELECTRON-TRANSFER

KW - CRYSTAL-STRUCTURE

KW - BINDING

KW - MECHANISM

U2 - 10.1021/bi200685w

DO - 10.1021/bi200685w

M3 - Article

SN - 0006-2960

VL - 50

SP - 6312

EP - 6315

JO - Biochemistry

JF - Biochemistry

IS - 29

ER -