Structure of the light harvesting antenna from Rhodospirillum molischianum studied by electron microscopy

Arjen F. Boonstra, Lothar Germeroth, Egbert J. Boekema

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Abstract

The structure of two types of isolated light-harvesting antenna complexes from Rhodospirillum molischianum was studied by electron microscopy and image analysis. The B870 reaction center complex forms an almost circular particle with a diameter in the plane of the membrane of about 10.7-11.2 nm. A complex consists of a reaction center surrounded most likely by 12 B870 antennae units. An asymmetrical protrusion, consisting of the cytochrome subunit, gives the reaction center a height of 11.5 nm. The peripheral B800-820 complex is cylindrical, with a diameter of 5.3 nm and a small central indentation. At low resolution, it is structurally very similar to the B800-850 complex of Rhodobacter sphaeroides and is considered to be most likely of the alpha(6) beta(6) type.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalBiochimica et Biophysica Acta
Volume1184
Issue number2-3
DOIs
Publication statusPublished - 8-Mar-1994

Keywords

  • LIGHT-HARVESTING COMPLEX
  • PHOTOSYNTHESIS
  • BACTERIUM
  • ELECTRON MICROSCOPY
  • (RHODOSPIRILLUM MOLISCHIANUM)
  • PHOTOSYNTHETIC REACTION CENTER
  • RHODOPSEUDOMONAS-VIRIDIS
  • COMPLEX
  • UNIT
  • CRYSTALLIZATION
  • PROTEINS
  • SUBUNITS

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