Structure-Specific Fermentation of Galacto-Oligosaccharides, Isomalto-Oligosaccharides and Isomalto/Malto-Polysaccharides by Infant Fecal Microbiota and Impact on Dendritic Cell Cytokine Responses

Madelon J Logtenberg, Renate Akkerman*, Rosan Hobe, Kristel M H Donners, Sander S Van Leeuwen, Gerben D A Hermes, Bart J de Haan, Marijke M Faas, Piet L Buwalda, Erwin G Zoetendal, Paul de Vos, Henk A Schols

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

SCOPE: Next to galacto-oligosaccharides (GOS), starch-derived isomalto-oligosaccharide preparation (IMO) and isomalto/malto-polysaccharides (IMMP) could potentially be used as prebiotics in infant formulas. However, it remains largely unknown how the specific molecular structures of these non-digestible carbohydrates (NDCs) impact fermentability and immune responses in infants.

METHODS AND RESULTS: In vitro fermentation of GOS, IMO and IMMP using infant fecal inoculum of 2- and 8-week-old infants showed that only GOS and IMO were fermented by infant fecal microbiota. The degradation of GOS and IMO coincided with an increase in Bifidobacterium and production of acetate and lactate, which was more pronounced with GOS. Individual isomers with an (1↔1)-linkage or di-substituted reducing terminal glucose residue were more resistant to fermentation. GOS, IMO and IMMP fermentation digesta attenuated cytokine profiles in immature dendritic cells (DCs), but the extent was dependent on the infants age and NDC structure.

CONCLUSION: The IMO preparation, containing reducing and non-reducing isomers, showed similar fermentation patterns as GOS in fecal microbiota of 2-week-old infants. Knowledge obtained on the substrate specificities of infant fecal microbiota and the subsequent regulatory effects of GOS, IMO and IMMP on DC responses might contribute to the design of tailored NDC mixtures for infants of different age groups. This article is protected by copyright. All rights reserved.

Original languageEnglish
Article numbere2001077
Pages (from-to)1-16
Number of pages16
JournalMolecular Nutrition & Food Research
DOIs
Publication statusPublished - 1-Jun-2021

Keywords

  • galacto-oligosaccharides
  • in vitro fermentation
  • infant formula
  • isomalto
  • malto-polysaccharides
  • isomalto-oligosaccharides
  • CHAIN FATTY-ACIDS
  • IN-VITRO
  • GUT MICROBIOTA
  • LACTOBACILLUS-REUTERI
  • DOUBLE-BLIND
  • FORMULA
  • STARCH
  • BIFIDOBACTERIA
  • IDENTIFICATION
  • IMMUNITY

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