Suppression of cdc13-2-associated senescence by pif1-m2 requires Ku-mediated telomerase recruitment

Enikő Fekete-Szücs, Fernando Rosas Bringas, Sonia Stinus, Michael Chang*

*Corresponding author for this work

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Abstract

In Saccharomyces cerevisiae, recruitment of telomerase to telomeres requires an interaction between Cdc13, which binds single-stranded
telomeric DNA, and the Est1 subunit of telomerase. A second pathway involving an interaction between the yKu complex and telomerase
RNA (TLC1) contributes to telomerase recruitment but cannot sufficiently recruit telomerase on its own to prevent replicative senescence
when the primary Cdc13-Est1 pathway is abolished—for example, in the cdc13-2 mutant. In this study, we find that mutation of PIF1,
which encodes a helicase that inhibits telomerase, suppresses the replicative senescence of cdc13-2 by increasing reliance on the
yKu-TLC1 pathway for telomerase recruitment. Our findings reveal new insight into telomerase-mediated telomere maintenance.
Original languageEnglish
Number of pages8
JournalG3 Genes|Genomes|Genetics
Volume12
Issue number1
DOIs
Publication statusPublished - 4-Jan-2022

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