Surface-modified nanoparticles as a new, versatile, and mechanically robust nonadhesive coating: Suppression of protein adsorption and bacterial adhesion

P. F. Holmes, E. P. K. Currie, J. C. Thies*, H. C. van der Mei, H. J. Busscher, W. Norde

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    41 Citations (Scopus)


    The synthesis of surface-modified silica nanoparticles, chemically grafted with acrylate and poly(ethylene glycol) (PEG) groups, and the ability of the resulting crosslinked coatings to inhibit protein adsorption and bacterial adhesion are explored. Water contact angles, nanoindentation, and atomic force microscopy were used to characterize the cross-linked coatings. Coatings showed a high degree of hydrophilicity combined with a remarkable hardness and stiffness in the dry state. Adsorption of the small protein lysozyme from buffer solution on coated silica wafers decreased significantly with increasing grafting density of the PEG groups on the nanoparticles and was completely inhibited at 0.6 chains nm(-2). Coatings significantly reduced adhesion of Staphylococcus epidermidis HBH 276 in a parallel plate flow chamber with respect to bare glass (>90%), whereas adhesion of Pseudomonas aeruginosa AK1 was only marginally affected by the presence of the coating (930%) of both strains from coated glass, indicating that the adhesion strength between both bacterial strains and the coated surface was significantly reduced by the grafted PEG groups. These coatings thus provide a new method to prepare mechanically robust films with nonadhesive properties that will be extremely useful for the design of biocompatible Surfaces in biomedical applications. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 824-833, 2009

    Original languageEnglish
    Pages (from-to)824-833
    Number of pages10
    JournalJournal of Biomedical Materials Research. Part A
    Issue number3
    Publication statusPublished - 1-Dec-2009


    • protein adsorption
    • bacterial adhesion
    • photopolymerization
    • nanoparticle
    • poly(ethylene glycol)
    • surface modification
    • nonadhesive
    • GLYCOL)
    • RESIST
    • MODEL
    • FLOW

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