Systematic expression analysis of plasticity-related genes in mouse brain development brings PRG4 into play

Isabel Gross, Tabea Tschigor, Angelina L. Salman, Fan Yang, Jiankai Luo, Danara Vonk, Mark S. Hipp, John Neidhardt, Anja U. Bräuer*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    7 Citations (Scopus)
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    Background: Plasticity-related genes (Prgs/PRGs) or lipid phosphate phosphatase-related proteins (LPPRs) comprise five known members, which have been linked to neuronal differentiation processes, such as neurite outgrowth, axonal branching, or dendritic spine formation. PRGs are highly brain-specific and belong to the lipid phosphate phosphatases (LPPs) superfamily, which influence lipid metabolism by dephosphorylation of bioactive lipids. PRGs, however, do not possess enzymatic activity, but modify lipid metabolism in a way that is still under investigation.

    Results: We analyzed mRNA expression levels of all Prgs during mouse brain development, in the hippocampus, neocortex, olfactory bulbs, and cerebellum. We found different spatio-temporal expression patterns for each of the Prgs, and identified a high expression of the uncharacterized Prg4 throughout brain development. Unlike its close family members PRG3 and PRG5, PRG4 did not induce filopodial outgrowth in non-neuronal cell lines, and does not localize to the plasma membrane of filopodia.

    Conclusion: We showed PRG4 to be highly expressed in the developing and the adult brain, suggesting that it is of vital importance for normal brain function. Despite its similarities to other family members, it seems not to be involved in changes of cell morphology; instead, it is more likely to be associated with intracellular signaling.

    Original languageEnglish
    Pages (from-to)714-728
    Number of pages15
    JournalDevelopmental dynamics
    Issue number4
    Early online date7-Oct-2021
    Publication statusPublished - Apr-2022


    • cell morphology
    • filopodia
    • mRNA expression
    • PRGs
    • primary brain cells

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