Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation

Nora A. Spraakman*, Annemieke M. Coester, Arno R. Bourgonje, Vincent B. Nieuwenhuijs, Jan-Stephan F. Sanders, Henri G. D. Leuvenink, Harry van Goor, Gertrude J. Nieuwenhuijs-Moeke

*Corresponding author for this work

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During ischemia-reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion (p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion (p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients.

Original languageEnglish
Article number9789
Number of pages11
JournalInternational Journal of Molecular Sciences
Issue number17
Publication statusPublished - 29-Aug-2022


  • ischemia-reperfusion injury
  • oxidative stress
  • free thiols
  • redox
  • kidney transplantation

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