TY - JOUR
T1 - Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation
AU - Spraakman, Nora A.
AU - Coester, Annemieke M.
AU - Bourgonje, Arno R.
AU - Nieuwenhuijs, Vincent B.
AU - Sanders, Jan-Stephan F.
AU - Leuvenink, Henri G. D.
AU - van Goor, Harry
AU - Nieuwenhuijs-Moeke, Gertrude J.
PY - 2022/8/29
Y1 - 2022/8/29
N2 - During ischemia-reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion (p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion (p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients.
AB - During ischemia-reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion (p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion (p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients.
KW - ischemia-reperfusion injury
KW - oxidative stress
KW - free thiols
KW - redox
KW - kidney transplantation
KW - REPERFUSION INJURY
KW - OXIDATIVE STRESS
KW - REACTIVE OXYGEN
KW - PLASMA
KW - THIOLS
U2 - 10.3390/ijms23179789
DO - 10.3390/ijms23179789
M3 - Article
SN - 1422-0067
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 17
M1 - 9789
ER -