Systemic anti-IFN-gamma treatment and role of macrophage subsets in the foreign body reaction to dermal sheep collagen in rats

I M S L Khouw, P B van Wachem, R J van der Worp, T K van den Berg, L F M H de Leij, M J A van Luyn

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    Abstract

    The application of a biomaterial induces a foreign body reaction. By controlling this reaction,biocompatibility could be improved. We previously demonstrated that impregnation of a biodegradable biomaterial with antibodies against interferon-gamma (IFN-gamma) inhibits the foreign body reaction. In this study we investigate whether systemic administration of the antibody can induce similar reactions. Several parameters are compared between control and anti-IFN-gamma-treated rats: cellular ingrowth; degradation of the biomaterial; ingrowth of macrophage (MO) subsets, T cells, B cells, NK cells, and granulocytes; and expression of the major histocompatibility complex class II (MHC class II) molecule on antigen presenting cells. Treatment with anti-IFN-gamma results in increased cellular ingrowth and biomaterial degradation and a decreased expression of MHC class II. Overall, systemic treatment with anti-IFN-gamma is insufficient to modulate the foreign body reaction. This suggests an alternative mechanism for MO activation besides IFN-gamma. The role of T cells and MO subsets in the foreign body reaction is discussed. (C) 2000 John Wiley & Sons, Inc.

    Original languageEnglish
    Pages (from-to)297-304
    Number of pages8
    JournalJournal of Biomedical Materials Research
    Volume49
    Issue number3
    DOIs
    Publication statusPublished - 5-Mar-2000

    Keywords

    • foreign body reaction
    • interferon-gamma
    • macrophages
    • major histocompatibility complex class II
    • MONOCLONAL-ANTIBODY
    • INTERFERON-GAMMA
    • TISSUE REACTION
    • INFLAMMATORY RESPONSES
    • MONOCYTES MACROPHAGES
    • CONSTANT DETERMINANT
    • LYMPHOID ORGANS
    • IMMUNE-RESPONSE
    • CROSS-LINKING
    • HETEROGENEITY

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