T-cell anergy induced by clonotype-specific antibodies: modulation of an autoreactive human T-cell clone in vitro

PGA Steenbakkers*, AMH Boots, AWM Rijnders

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Monoclonal antibodies (mAb) specific for the clonotype of an autoreactive T cell may be useful reagents in the modulation of autoimmune disease. We have previously reported the generation of a set of mAb specific for the clonotypic structure of a human T-cell clone recognizing an epitope of human cartilage gp-39. This glycoprotein was recently identified as a candidate autoantigen in rheumatoid arthritis. Here, we demonstrate for the first time that small amounts of immobilized anticlonotype mAb can induce anergy in the autoreactive clone. Following the anergic stimulus, T cells failed to proliferate upon restimulation as a result of a lack of interleukin-2, (IL-2) gene transcription. In addition, a diminished interferon-gamma (IFN-gamma) production was found. Our data indicate that anergy was not a result of T-cell receptor (TCR) downmodulation or the absence of free TCR. The anergic state was induced independent of costimulation or the presence of IL-2 and no protein synthesis was required for the induction of anergy. Anticlonotype mAb-induced anergy was prevented by cyclosporin A, suggesting that active signalling via the calcium/ calcineurin pathway was required for the induction of anergy. In coculture experiments, anergic T cells were found to suppress the response of reactive cells from the same clone. This bystander suppression led to 90% inhibition of peptide-induced proliferation. Together, these findings suggest that mAb to the clonotypic structure of autoreactive T cells may be suitable reagents for the functional inactivation of these T cells in autoimmune diseases.

Original languageEnglish
Pages (from-to)586-594
Number of pages9
JournalImmunology
Volume96
Issue number4
Publication statusPublished - Apr-1999

Keywords

  • ANTIGEN-PRESENTING CELLS
  • MONOCLONAL-ANTIBODY
  • IN-VITRO
  • MULTIPLE-SCLEROSIS
  • LYMPHOCYTES-T
  • RECEPTOR
  • INDUCTION
  • UNRESPONSIVENESS
  • ACTIVATION
  • TOLERANCE

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