TAK1 activation of the mouse JunB promoter is mediated through a CCAAT box and NF-Y

BJL Eggen*, GFJD Benus, S Folkertsma, LJ Jonk, W Kruijer

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The JunB gene is activated by many stimuli including transforming growth factor beta (TGF beta) family members and interleukin-6 (IL-6). Here the effect of TGFP activated kinase 1 (TAK1), a mitogen activated protein kinase kinase kinase (MAPKKK) implicated in TGF beta, bone morphogenetic protein (BMP) and interleukin-1 (IL-1) signaling, on JunB promoter activity was investigated. Promoter analysis led to the identification of a CCAAT motif in the JunB gene, essential for activation by TAK1. Transfer of this CCAAT element to a heterologous minimal promoter conferred TAK1-responsiveness. The CCAAT-binding transcription factor, nuclear factor Y (NF-Y), activated the JunB promoter and a dominant negative NF-YA construct inhibited TAK1 activation of JunB. Our results demonstrate that JunB gene activation by TAK1 is mediated by the CCAAT-binding factor NF-Y. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalFEBS Letters
Volume506
Issue number3
DOIs
Publication statusPublished - 12-Oct-2001

Keywords

  • JunB
  • transforming growth factor beta activated kinase 1
  • nuclear factor Y
  • promoter
  • CCAAT motif
  • SIGNAL-TRANSDUCTION PATHWAY
  • MAP KINASE CASCADE
  • GROWTH-FACTOR-BETA
  • ELEMENT
  • PROTEIN
  • IDENTIFICATION
  • TRANSCRIPTION
  • TAB1

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