Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Jonas Lohse; Alexandra Schindl; Natasha Danda; Chris P Williams; Karl Kramer; Bernhard Kuster; Martin D Witte; Guillaume Médard

doi:10.1039/C7CC07001B

Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Jonas Lohse, Alexandra Schindl, Natasha Danda, Chris P Williams, Karl Kramer, Bernhard Kuster, Martin D Witte^*, Guillaume Médard^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Original language	English
Pages (from-to)	11929-11932
Number of pages	4
Journal	Chem. Commun.
Volume	53
Issue number	87
DOIs	https://doi.org/10.1039/C7CC07001B
Publication status	Published - 11-Nov-2017

Keywords

AZIDE-ALKYNE CYCLOADDITION
CHEMICAL PROTEOMICS
BIOORTHOGONAL CHEMISTRY
BIOTIN LIGASE
ENRICHMENT
BIOCONJUGATION
REPORTERS
MIXTURES
RELEASE
BIOLOGY

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10.1039/C7CC07001B

Target and identifyFinal publisher's version, 692 KBLicence: Taverne

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Cite this

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title = "Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy",

abstract = "A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.",

keywords = "AZIDE-ALKYNE CYCLOADDITION, CHEMICAL PROTEOMICS, BIOORTHOGONAL CHEMISTRY, BIOTIN LIGASE, ENRICHMENT, BIOCONJUGATION, REPORTERS, MIXTURES, RELEASE, BIOLOGY",

author = "Jonas Lohse and Alexandra Schindl and Natasha Danda and Williams, {Chris P} and Karl Kramer and Bernhard Kuster and Witte, {Martin D} and Guillaume M{\'e}dard",

year = "2017",

month = nov,

day = "11",

doi = "10.1039/C7CC07001B",

language = "English",

volume = "53",

pages = "11929--11932",

journal = "Chem. Commun.",

issn = "1359-7345",

publisher = "ROYAL SOC CHEMISTRY",

number = "87",

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TY - JOUR

T1 - Target and identify

T2 - triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

AU - Lohse, Jonas

AU - Schindl, Alexandra

AU - Danda, Natasha

AU - Williams, Chris P

AU - Kramer, Karl

AU - Kuster, Bernhard

AU - Witte, Martin D

AU - Médard, Guillaume

PY - 2017/11/11

Y1 - 2017/11/11

N2 - A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

AB - A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

KW - AZIDE-ALKYNE CYCLOADDITION

KW - CHEMICAL PROTEOMICS

KW - BIOORTHOGONAL CHEMISTRY

KW - BIOTIN LIGASE

KW - ENRICHMENT

KW - BIOCONJUGATION

KW - REPORTERS

KW - MIXTURES

KW - RELEASE

KW - BIOLOGY

U2 - 10.1039/C7CC07001B

DO - 10.1039/C7CC07001B

M3 - Article

C2 - 29046906

SN - 1359-7345

VL - 53

SP - 11929

EP - 11932

JO - Chem. Commun.

JF - Chem. Commun.

IS - 87

ER -