Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Jonas Lohse, Alexandra Schindl, Natasha Danda, Chris P Williams, Karl Kramer, Bernhard Kuster, Martin D Witte, Guillaume Médard

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Original languageEnglish
Pages (from-to)11929-11932
Number of pages4
JournalChem. Commun.
Volume53
Issue number87
DOIs
Publication statusPublished - 11-Nov-2017

Keywords

  • AZIDE-ALKYNE CYCLOADDITION
  • CHEMICAL PROTEOMICS
  • BIOORTHOGONAL CHEMISTRY
  • BIOTIN LIGASE
  • ENRICHMENT
  • BIOCONJUGATION
  • REPORTERS
  • MIXTURES
  • RELEASE
  • BIOLOGY

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