Targeted diazotransfer to proteins

Jonas Lohse

Research output: ThesisThesis fully internal (DIV)

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Abstract

Historically, the chemical sciences distinguish between those compounds that are organic, carbon-based molecules, and those that are inorganic, such as salts or metals.
A second distinction is based on the fact that some of these ions and molecules exist in the living world, i.e. inside cells and whole organisms (biotic) and the remainder that does not (xenobiotic). These two classifications overlap but they are not identical. For instance, table salt is an inorganic compound, yet it is responsible for signal transduction in our nervous system. Some drugs made in the laboratory are carbon-based but they do not occur naturally.
Curiosity is an inherent property to all human beings, and so the understanding of the ever so complex living world on a molecular basis has been an object of intense investigations for chemical biologists.
Throughout the past two decades a new methodological trick to study the living world has been brought forward: chemical groups that do not occur there naturally provide an entry point to study molecular mechanisms that previously were challenging to analyze. Often, these analyses were limited to methods that apply radioactivity. The novel methodologies replace these hazardous procedures and coupled with further evaluation by fluorescence imaging and mass spectrometry provide an even deeper insight into the process. The main objective of this thesis is to establish new methods for the introduction of such xenobiotic groups, specifically the azide, via small molecule probes to proteins. This selectively introduced group is then used for further studies of the labelled proteins in complex experimental settings.
Translated title of the contributionligand-gestuurde diazotransfer naar eiwitten
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
Supervisors/Advisors
  • Minnaard, Adriaan, Supervisor
  • Witte, Martin, Supervisor
Award date8-Jun-2018
Place of Publication[Groningen]
Publisher
Print ISBNs978-94-034-0733-3
Electronic ISBNs978-94-034-0732-6
Publication statusPublished - 2018

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