Targeting of liposomes to sinusoidal liver cells: Massive uptake by liver endothelial cells via a scavenger receptor mediated process

JAAM Kamps, HWM Morselt, PJ Swart, DKF Meijer, GL Scherphof

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Human serum albumin (HSA) derivatized with cis-aconitic anhydride (Aco-HSA) was covalently coupled to liposomes (Aco-HSA-Lip). Aco-HSA-Lip were cleared from the blood and taken up by the liver within 30 minutes after injection into a rat. In the liver especially endothelial cells and to a lesser extent Kupffer cells were responsible for this uptake, which could be inhibited by preinjection with poly inosinic acid. Binding and competition experiments with cultured liver endothelial and Kupffer cells revealed that the uptake of Aco-HSA-Lip was mediated by scavenger receptors. Aco-HSA-Lip may prove to be useful drugcarriers for various liver or liver endothelium associated disorders.

Original languageEnglish
Title of host publicationCELLS OF THE HEPATIC SINUSOID, VOL 6
EditorsE Wisse, DL Knook, C Balabaud
Place of PublicationLEIDEN
PublisherThe Kupffer Cell Foundation
Pages387-388
Number of pages2
ISBN (Print)90-803856-1-1
Publication statusPublished - 1997
Event8th International Symposium on the Cells of the Hepatic Sinusoid - , France
Duration: 1-Sep-19965-Sep-1996

Other

Other8th International Symposium on the Cells of the Hepatic Sinusoid
CountryFrance
Period01/09/199605/09/1996

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